Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	784,602	A→G	P74P (CCT→CCC)	zitB ←	Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	784,602	0	A	G	100.0%	59.8 / NA	18	P74P (CCT→CCC)	zitB	Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/7);  total (11/7)

ACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCC  >  NC_000913/784468‑784736                                                                                                                                       |                                                                                                                                       aCCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGgac                                                                                                                                    <  1:264030/139‑1 (MQ=255) aCCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGgac                                                                                                                                    >  2:264030/1‑139 (MQ=255)                                    ccAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAg                                                                                                  >  1:224515/1‑138 (MQ=255)                                    ccAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAg                                                                                                  <  2:224515/138‑1 (MQ=255)                                       gACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAggg                                                                                             >  2:154491/1‑140 (MQ=255)                                          aaTCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGggcgg                                                                                          <  1:187223/140‑1 (MQ=255)                                                                    cTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCa                                                                >  2:147044/1‑140 (MQ=255)                                                                      aTGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATc                                                              >  2:251540/1‑140 (MQ=255)                                                                            ttCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAg                                                        >  1:266051/1‑140 (MQ=255)                                                                                 aaaCGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATg                                                   >  1:31123/1‑140 (MQ=255)                                                                                         cgAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAg                                                        <  2:52586/127‑1 (MQ=255)                                                                                         cgAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAg                                                        >  1:52586/1‑127 (MQ=255)                                                                                                      tCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAac                              >  1:81427/1‑140 (MQ=255)                                                                                                          aGCCAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAaccacc                          >  2:175848/1‑140 (MQ=255)                                                                                                            ccAACCGAAAGTGTGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCaa                        >  2:182895/1‑140 (MQ=255)                                                                                                                      gtgtGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTAc              <  2:81427/140‑1 (MQ=255)                                                                                                                      gtgtGGCGAATGGTGGGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTAc              <  1:154491/140‑1 (MQ=255)                                                                                                                                  gtggGGGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAAccc  <  1:182895/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAGCAGGGCGGCAGTATCGGTCAACATATGACCCGCATCGGCCAGCAATGCCAGAGAACCAGAAAGAAAACCACCAACGACTTCTACCAGCATAAACCC  >  NC_000913/784468‑784736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑