Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	801,638	A→G	T294A (ACC→GCC)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	801,638	0	A	G	100.0%	103.6 / NA	30	T294A (ACC→GCC)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

CTAAACCTGTGCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGAC  >  NC_000913/801504‑801771                                                                                                                                       |                                                                                                                                      cTAAACCTGTGCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTc                                                                                                                                  <  2:352244/140‑1 (MQ=255)          tgCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACaa                                                                                                                         <  1:137760/140‑1 (MQ=255)          tgCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACaa                                                                                                                         >  2:206391/1‑140 (MQ=255)          tgCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACaa                                                                                                                         >  1:88188/1‑140 (MQ=255)               aTTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGt                                                                                                                    <  2:88188/140‑1 (MQ=255)               aTTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGt                                                                                                                    <  1:172112/140‑1 (MQ=255)                      cAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCt                                                                                                             >  1:137163/1‑140 (MQ=255)                         cgcAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTa                                                                                                          >  1:205536/1‑140 (MQ=255)                           cAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAgg                                                                                                        >  2:194147/1‑140 (MQ=255)                                  gCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                 <  1:194147/140‑1 (MQ=255)                                  gCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGc                                                                                                 <  2:137163/140‑1 (MQ=255)                                                 tgCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                  <  1:324959/140‑1 (MQ=255)                                                 tgCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                  <  2:265966/140‑1 (MQ=255)                                                 tgCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATAGTGaa                                                                                  >  2:326843/1‑140 (MQ=255)                                                  gCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAac                                                                                 <  2:205536/140‑1 (MQ=255)                                                   cGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGACACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAaca                                                                                <  2:33682/140‑1 (MQ=255)                                                             tGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAgtggt                                                                      >  1:45508/1‑140 (MQ=255)                                                                  cATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                  <  1:340662/123‑1 (MQ=255)                                                                  cATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGaa                                                                                  >  2:340662/1‑123 (MQ=255)                                                                       ttGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACg                                                            <  1:326843/140‑1 (MQ=255)                                                                              cgcgcgCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCAttt                                                     >  1:76254/1‑140 (MQ=255)                                                                                                 gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAAt                                              <  2:106421/128‑1 (MQ=255)                                                                                                 gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAAt                                              >  1:106421/1‑128 (MQ=255)                                                                                                 gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGt                                        <  1:77344/134‑1 (MQ=255)                                                                                                 gTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGt                                        >  2:77344/1‑134 (MQ=255)                                                                                                               tCCAGTAGTTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTAcg                    <  2:45508/140‑1 (MQ=255)                                                                                                                    tagtTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCAt               >  2:100523/1‑140 (MQ=255)                                                                                                                    tagtTGTGCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATCCCACGACGTTAGGCGCAt               >  2:39376/1‑140 (MQ=255)                                                                                                                         gtgCATTGGAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGtt          >  2:30174/1‑140 (MQ=255)                                                                                                                                 gAAGGCGCCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGgac  >  2:353599/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      CTAAACCTGTGCTTATTTCTCCAGCGCAGAAAGGCGGGGCAATTAATGTGCGTTATTTTATGCCGCATTCTTGCCATCGCGCGCTGGCGATAACCGGTGCTATTGCTATTTCCAGTAGTTGTGCATTGGAAGGCACCGTCACCCGACAAATCGTCCCTTCTGTAGGATACGGCAATATCAATATTGAACACCCCAGTGGTGCGCTCGACGTTCATTTAAGTAATGAAGGTCAGGATGCCACGACGTTACGCGCATCTGTTATTCGGAC  >  NC_000913/801504‑801771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑