Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,675	C→T	D283N (GAT→AAT)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,675	0	C	T	100.0%	58.2 / NA	18	D283N (GAT→AAT)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/11);  total (7/11)

CACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGC  >  NC_000913/73544‑73801                                                                                                                                    |                                                                                                                               cACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGt                                                                                                                        >  2:94458/1‑140 (MQ=255) cACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGt                                                                                                                        >  1:303880/1‑140 (MQ=255)           gCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGcc                                                                                                              >  1:160059/1‑140 (MQ=255)                             tGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGcc                                                                                            >  2:141752/1‑140 (MQ=255)                                    ttACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCa                                                                                     <  1:321445/140‑1 (MQ=255)                                    ttACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCa                                                                                     <  1:94458/140‑1 (MQ=255)                                     ccccccACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCaa                                                                                    <  2:75289/138‑1 (MQ=255)                                        cccACCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCATGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATgg                                                                                 <  2:283146/140‑1 (MQ=255)                                           aTCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCt                                                                              <  2:160059/140‑1 (MQ=255)                                                       cGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCt                                                                  <  1:331113/140‑1 (MQ=255)                                                         ccAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGa                                                                <  1:141752/140‑1 (MQ=255)                                                                    ggCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCAc                                                     <  1:61955/140‑1 (MQ=255)                                                                                     cgcCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGcaca                                    <  1:230099/140‑1 (MQ=255)                                                                                                         tgtCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGc                >  2:257573/1‑140 (MQ=255)                                                                                                          gtCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCa               >  1:209768/1‑140 (MQ=255)                                                                                                                aaTGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcg         <  1:16300/140‑1 (MQ=255)                                                                                                                   gCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCcgcgc      >  1:152428/1‑140 (MQ=255)                                                                                                                       cTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGc  <  2:152428/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                               CACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGATCACCGCCAGTAACGGTGGCAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGC  >  NC_000913/73544‑73801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑