Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,194	A→G	Y148C (TAC→TGC)	ycbJ →	putative phosphotransferase YcbJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,194	0	A	G	100.0%	45.4 / NA	14	Y148C (TAC→TGC)	ycbJ	putative phosphotransferase YcbJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/6);  total (8/6)

ACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTACCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGACGATGCAGGATAAGCGGATCCTGTTTCGCACTCGCGAATGTCTCCCGGCATTATTTGAAGG  >  NC_000913/972057‑972314                                                                                                                                          |                                                                                                                         aCCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGcc                                                                                                                        >  2:200804/1‑140 (MQ=255)             acCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTg                                                                                                            <  1:200804/140‑1 (MQ=255)               cAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGaa                                                                                                          <  2:295898/140‑1 (MQ=255)                                    aaGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAAt                                                                                     <  2:270340/140‑1 (MQ=255)                                                     gCCTTACTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCgg                                                                    <  1:301361/140‑1 (MQ=255)                                                          aCTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAacac                                                                       >  1:85367/1‑132 (MQ=255)                                                          aCTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAacac                                                                       <  2:85367/132‑1 (MQ=255)                                                                   gcACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGa                                                               <  1:223109/131‑1 (MQ=255)                                                                   gcACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGa                                                               >  2:223109/1‑131 (MQ=255)                                                                        gTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGACGATGCAGGATAAg                                                 >  2:67293/1‑140 (MQ=255)                                                                                  tCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGACGATGCAGGATAAGCGGATCCTGt                                       >  1:252893/1‑140 (MQ=255)                                                                                                                  aGGAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGACGATGCAGGATAAGCGGATCCTGTTTCGCACTCGCGAATGTCTCCCGGCATTAttt       >  2:210816/1‑140 (MQ=255)                                                                                                                    gAAAATTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGACGATGCAGGATAAGCGGATCCTGTTTCGCACTCGCGAATGTCTCCCGGCATTATTTGa     >  2:94955/1‑140 (MQ=255)                                                                                                                       aaTTTCTGGCCCTCATGGTGCCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGACGATGCAGGATAAGCGGATCCTGTTTCGCACTCGCGAATGTCTCCCGGCATTATTTGAAgg  >  2:345908/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                         ACCAGCCCGAACACCAGAACGCTGGGAACAACTCAAAGACCAAATCGTTGAAGCCTTACTGGCCTGGCACCGTCAGGACAGTCGCGGTTGCGTCGGCGCGGTCGACAATACTCAGGAAAATTTCTGGCCCTCATGGTACCGGCAACATGTTGAAGTGCTATGGACCACGCTCAATCAGTTCAATAACACCGGTCTGACGATGCAGGATAAGCGGATCCTGTTTCGCACTCGCGAATGTCTCCCGGCATTATTTGAAGG  >  NC_000913/972057‑972314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑