Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,012,766	A→G	T256A (ACA→GCA)	pqiA →	intermembrane transport protein PqiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,012,766	0	A	G	100.0%	93.1 / NA	27	T256A (ACA→GCA)	pqiA	intermembrane transport protein PqiA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

AGGCGTCACGGGGATACGTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGACACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAA  >  NC_000913/1012636‑1012897                                                                                                                                   |                                                                                                                                    aGGCGTCACGGGGATACGTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTcgcg                                                                                                                             >  2:201126/1‑139 (MQ=255) aGGCGTCACGGGGATACGTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTcgcg                                                                                                                             <  1:201126/139‑1 (MQ=255) aGGCGTCACGGGGATACGTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCgcgc                                                                                                                            <  1:168889/140‑1 (MQ=255) aGGCGTCACGGGGATACGTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCgcgc                                                                                                                            <  1:50992/140‑1 (MQ=255)    cGTCACGGGGATACGTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCgctgc                                                                                                                         >  2:327782/1‑140 (MQ=255)                 cGTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCcat                                                                                                            <  1:76980/140‑1 (MQ=255)                  gTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCcatc                                                                                                           <  2:192696/140‑1 (MQ=255)                  gTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCcatc                                                                                                           <  1:37498/140‑1 (MQ=255)                           tGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTAt                                                                                                  >  1:249200/1‑140 (MQ=255)                            gCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATc                                                                                                 >  2:188164/1‑140 (MQ=255)                              gTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCtt                                                                                               <  1:123342/140‑1 (MQ=255)                                             gtACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGc                                                                                          <  1:323675/130‑1 (MQ=255)                                             gtACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGc                                                                                          >  2:323675/1‑130 (MQ=255)                                                   cAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGcc                                                                              >  2:106554/1‑136 (MQ=255)                                                   cAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGcc                                                                              <  1:106554/136‑1 (MQ=255)                                                     aTCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCcatcat                                                                        >  2:281367/1‑140 (MQ=255)                                                          tcctGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGa                                                                   <  2:354444/140‑1 (MQ=255)                                                                     aaCCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATttatta                                                        <  1:327782/140‑1 (MQ=255)                                                                                          gtaCCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTcgacg                                   >  1:184790/1‑140 (MQ=255)                                                                                              cAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATtc                               >  1:311052/1‑140 (MQ=255)                                                                                                      aCGTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTggg                       >  2:259835/1‑140 (MQ=255)                                                                                                        gTTCGGCGTAGAAACAGCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGt                     <  2:311052/140‑1 (MQ=255)                                                                                                                        gCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCa                   >  2:141176/1‑126 (MQ=255)                                                                                                                        gCCTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCa                   <  1:141176/126‑1 (MQ=255)                                                                                                                          cTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGa   >  2:249691/1‑140 (MQ=255)                                                                                                                          cTGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGa   >  2:268326/1‑140 (MQ=255)                                                                                                                           tGCAGTGGGCACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGaa  <  1:281367/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    AGGCGTCACGGGGATACGTCAGGGGCTGCGTTCGTGCTCCTGTTGTACGGCAATCCTTCCTGCTGATGAACCCGTGTGCCCGCGTTGTAGTACCAAAGGGTACGTTCGGCGTAGAAACAGCCTGCAGTGGACACTCGCGCTGCTTGTAACGTCCATCATGCTGTATCTTCCGGCTAATATTTTGCCCATCATGGTGACGGATTTATTAGGCTCGAAGATGCCGTCGACGATTCTCGCTGGGGTCATTCTGTTATGGAGCGAA  >  NC_000913/1012636‑1012897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑