Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,064,754	A→G	N240S (AAC→AGC)	yccE →	uncharacterized protein YccE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,754	0	A	G	100.0%	74.1 / NA	22	N240S (AAC→AGC)	yccE	uncharacterized protein YccE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/11);  total (11/11)

ACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAG  >  NC_000913/1064622‑1064886                                                                                                                                     |                                                                                                                                     aCCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTc                                                                                                                               >  1:120943/1‑140 (MQ=255)                       tGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATaaaaaaa                                                                                                         >  2:348215/1‑140 (MQ=255)                                aGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAgg                                                                                                <  1:316432/140‑1 (MQ=255)                                aGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAgg                                                                                                <  1:49756/140‑1 (MQ=255)                                    ggCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGctct                                                                                            >  2:216413/1‑140 (MQ=255)                                    ggCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGctct                                                                                            >  2:313355/1‑140 (MQ=255)                                         tGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCttgttg                                                                                       >  2:122880/1‑140 (MQ=255)                                                tGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGc                                                                                <  1:216413/140‑1 (MQ=255)                                                 gTCTGTTAAGAGCTATACTTGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCa                                                                               <  2:235947/140‑1 (MQ=255)                                                  tCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCAt                                                                              <  1:303249/140‑1 (MQ=255)                                                        aaGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCtata                                                                        <  2:120943/140‑1 (MQ=255)                                                                taCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAgg                                                                >  1:182251/1‑140 (MQ=255)                                                                taCTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAgg                                                                <  1:159580/140‑1 (MQ=255)                                                                                             tGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGa                                   <  1:313355/140‑1 (MQ=255)                                                                                              gggCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCg                                       >  1:188124/1‑135 (MQ=255)                                                                                              gggCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCg                                       <  2:188124/135‑1 (MQ=255)                                                                                              gggCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATACTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGAt                                  >  2:329440/1‑140 (MQ=255)                                                                                                                   tGCAAAAGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAAtt             >  1:204738/1‑140 (MQ=255)                                                                                                                     aaaaaGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGCAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCa           >  2:60032/2‑140 (MQ=255)                                                                                                                         aGGAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAgggg       <  1:119659/140‑1 (MQ=255)                                                                                                                           gAAGTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCa     >  2:200874/1‑140 (MQ=255)                                                                                                                              gTTTACAGCGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCagag  <  1:69673/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     ACCAGGCATTAGGATTAAAATATGCTCCTGTAGATGGCGATGGATATTGTCTGTTAAGAGCTATACTGGTTTTAAAACAACATGATTATTCATGGGCGCTGGTCAGTTATAAGATGCAAAAGGAAGTTTACAACGAATTCATTAAAATGGTTGATAAAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAG  >  NC_000913/1064622‑1064886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑