Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,070,255	A→G	F34L (TTC→CTC)	rutF ←	flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,070,255	0	A	G	100.0%	97.7 / NA	29	F34L (TTC→CTC)	rutF	flavin reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/12);  total (17/12)

AAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCT  >  NC_000913/1070133‑1070386                                                                                                                           |                                                                                                                                    aaGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTg                                                                                                                    >  2:16215/1‑140 (MQ=255)                caGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCgtg                                                                                                         >  1:221397/1‑136 (MQ=255)                caGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCgtg                                                                                                         <  2:221397/136‑1 (MQ=255)                  gCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGACTGGACCGTCCGTGGTGata                                                                                                   >  1:342343/1‑140 (MQ=255)                   cGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGa                                                                                                                  >  2:57607/1‑124 (MQ=255)                   cGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGa                                                                                                                  <  1:57607/124‑1 (MQ=255)                   cGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGataa                                                                                                  >  1:28154/1‑140 (MQ=255)                   cGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGataa                                                                                                  >  1:84855/1‑140 (MQ=255)                   cGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGCGataa                                                                                                  >  1:177892/1‑140 (MQ=255)                      tCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAatat                                                                                               >  1:266886/1‑140 (MQ=255)                            ttCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGAccg                                                                                         <  2:266886/140‑1 (MQ=255)                                ttGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACcgccgc                                                                                     <  2:84855/140‑1 (MQ=255)                                  gAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCgcgc                                                                                   <  1:16215/140‑1 (MQ=255)                                          gCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCgcgc                                                                                   <  1:278422/132‑1 (MQ=255)                                          gCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCgcgc                                                                                   >  2:278422/1‑132 (MQ=255)                                                               tCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGtt                                                      <  2:177892/140‑1 (MQ=255)                                                                 aGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTttg                                                    >  1:131598/1‑140 (MQ=255)                                                                                 tgGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTc                                    >  2:165307/1‑140 (MQ=255)                                                                                 tgGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCACGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTc                                    >  1:49905/1‑140 (MQ=255)                                                                                    gcggcgTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATa                                 >  2:163040/1‑140 (MQ=255)                                                                                         gTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTg                            <  2:28154/140‑1 (MQ=255)                                                                                         gTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTg                            <  1:163040/140‑1 (MQ=255)                                                                                         gTATCGGTCACACTGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGACTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCACATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTg                            <  2:342343/140‑1 (MQ=255)                                                                                                      tGCAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAg               >  1:78222/1‑140 (MQ=255)                                                                                                        cAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTacaaca                >  1:293352/1‑137 (MQ=255)                                                                                                        cAGACGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTacaaca                <  2:293352/137‑1 (MQ=255)                                                                                                            cGGCGCTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCa         <  2:131598/140‑1 (MQ=255)                                                                                                              gcgcTGGCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAgg       >  2:264552/1‑140 (MQ=255)                                                                                                                   ggCGGTGAGCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGcttct  >  2:322585/1‑140 (MQ=255)                                                                                                                           |                                                                                                                                    AAGTGTATTTACACACAGCGTTCGATTTTCATTGAATGCCGGCCAGACGGACGCCCCACGATTCAGGCACACCAGTAATGTGGGCGGCGTATCGGTCACACTGCAGACGGCGCTGGCGGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCT  >  NC_000913/1070133‑1070386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑