Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,108,598	C→T	G115E (GGA→GAA)	opgC ←	protein required for succinyl modification of osmoregulated periplasmic glucans

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,108,598	0	C	T	100.0%	59.0 / NA	18	G115E (GGA→GAA)	opgC	protein required for succinyl modification of osmoregulated periplasmic glucans
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/2);  total (16/2)

ACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAA  >  NC_000913/1108481‑1108719                                                                                                                      |                                                                                                                          aCGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCataat                                                                                                     >  2:86084/1‑140 (MQ=255)      caGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAAtt                                                                                                >  2:213292/1‑140 (MQ=255)       aGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTg                                                                                               >  1:295547/1‑140 (MQ=255)         cgtcgtCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCg                                                                                             >  1:9736/1‑140 (MQ=255)                                gTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATATAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCt                                                                      >  1:331450/1‑140 (MQ=255)                                      aCCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAAc                                                                >  2:188073/1‑140 (MQ=255)                                                        aTTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGt                                              <  2:9736/140‑1 (MQ=255)                                                        aTTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGt                                              >  2:6415/1‑140 (MQ=255)                                                                             atTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCAt                         <  1:86084/140‑1 (MQ=255)                                                                                 gTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCAttttt                     >  2:255188/1‑140 (MQ=255)                                                                                     tACAATGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCaa                 >  2:151194/1‑140 (MQ=255)                                                                                          tGACAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGAt            >  2:354611/1‑140 (MQ=255)                                                                                            aCAGCCCAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAg          >  2:336657/1‑140 (MQ=255)                                                                                                cccAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTa      >  2:102315/1‑140 (MQ=255)                                                                                                cccAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTa      >  2:320350/1‑140 (MQ=255)                                                                                                cccAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTa      >  1:151327/1‑140 (MQ=255)                                                                                                cccAGGCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTa      >  1:109702/1‑140 (MQ=255)                                                                                                    ggCCAACTTTCCGCTTTTTCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTaaaaa  >  1:112512/1‑140 (MQ=255)                                                                                                                      |                                                                                                                          ACGCACAGCGTCGTCATGACCACTAACACCAGTAAAAACCACAGGTGTGATATTAATTCCCAGGCCAACGTATTATATTTGTCATACAATGACAGCCCAGGCCAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAAA  >  NC_000913/1108481‑1108719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑