Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,134,318	C→T	R173C (CGC→TGC)	flgF →	flagellar basal‑body rod protein FlgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,134,318	0	C	T	100.0%	72.8 / NA	22	R173C (CGC→TGC)	flgF	flagellar basal‑body rod protein FlgF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

TGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCA  >  NC_000913/1134187‑1134449                                                                                                                                    |                                                                                                                                    tgCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTgac                                                                                                                             >  1:61905/1‑140 (MQ=255)                     aCTATTGCTGCCGATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAg                                                                                                         >  2:296734/1‑140 (MQ=255)                           gctgcCGATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGa                                                                                                   <  1:173875/140‑1 (MQ=255)                              gcCGATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAAc                                                                                                >  1:109032/1‑140 (MQ=255)                              gcCGATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAAc                                                                                                >  1:285952/1‑140 (MQ=255)                                 gATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCa                                                                                             >  2:206425/1‑140 (MQ=255)                                    ggCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGc                                                                                          <  1:206425/140‑1 (MQ=255)                                                 cgcTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCg                                                                             <  1:318122/140‑1 (MQ=255)                                                 cgcTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCg                                                                             <  1:171390/140‑1 (MQ=255)                                                    tCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTa                                                                          <  2:285952/140‑1 (MQ=255)                                                                aTCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGAt                                                              >  2:220430/1‑140 (MQ=255)                                                                 tCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTgcaggcatctc                                                             <  1:217836/140‑5 (MQ=255)                                                                              gTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCgtgt                                                <  1:296734/140‑1 (MQ=255)                                                                                         aGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCggggg                                     <  2:162695/140‑1 (MQ=255)                                                                                             cGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGtgtg                                               >  1:307217/1‑126 (MQ=255)                                                                                             cGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGtgtg                                               <  2:307217/126‑1 (MQ=255)                                                                                             cGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGGACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCt                                 >  2:331522/1‑140 (MQ=255)                                                                                               tCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCgg                                        <  2:92784/131‑1 (MQ=255)                                                                                               tCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCgg                                        >  1:92784/1‑131 (MQ=255)                                                                                                  tAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGa                              >  2:223643/1‑138 (MQ=255)                                                                                                  tAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGa                              <  1:223643/138‑1 (MQ=255)                                                                                                                            aaGTGCAGTGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCa  <  2:61905/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    TGCCGGAAGGGGCGGAAATCACTATTGCTGCCGATGGCACAATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCA  >  NC_000913/1134187‑1134449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑