Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,136,668	A→G	Q132R (CAG→CGG)	flgI →	flagellar P‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,136,668	0	A	G	100.0%	83.6 / NA	24	Q132R (CAG→CGG)	flgI	flagellar P‑ring protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/10);  total (14/10)

AGCGTCACTTCCTCCGTTTGGACGTCAGGGGCAAACCATCGATGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCAGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCGGTTATTGAACGTGAATTGCCCAGC  >  NC_000913/1136543‑1136804                                                                                                                              |                                                                                                                                         aGCGTCACTTCCTCCGTTTGGACGTCAGGGGCAAACCATCGATGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTgg                                                                                                                            >  1:121578/1‑140 (MQ=255)   cGTCACTTCCTCCGTTTGGACGTCAGGGGCAAACCATCGATGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGgcg                                                                                                                          >  1:164362/1‑140 (MQ=255)      cACTTCCTCCGTTTGGACGTCAGGGGCAAACCATCGATGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAg                                                                                                                       >  1:299300/1‑140 (MQ=255)                                  aaCCATCGATGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAg                                                                                           >  1:231839/1‑140 (MQ=255)                                          aTGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCt                                                                                   <  2:164362/140‑1 (MQ=255)                                                    ttcttcCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCa                                                                         >  1:144512/1‑140 (MQ=255)                                                       ttcCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAgtg                                                                      <  2:231839/140‑1 (MQ=255)                                                             gggAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAgg                                                                >  2:198637/1‑140 (MQ=255)                                                                aaaTGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTa                                                             <  2:299300/140‑1 (MQ=255)                                                                                cGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTgg                                             >  1:140489/1‑140 (MQ=255)                                                                                           gttgttGATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCa                                  <  2:140489/140‑1 (MQ=255)                                                                                               ttgATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATgg                              <  1:340456/140‑1 (MQ=255)                                                                                               ttgATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATgg                              <  2:144512/140‑1 (MQ=255)                                                                                               ttgATGACACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCCGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATgg                              <  2:329470/140‑1 (MQ=255)                                                                                                 gatgaCACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTg                            <  1:121084/140‑1 (MQ=255)                                                                                                 gatgaCACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTg                            >  2:212746/1‑140 (MQ=255)                                                                                                 gatgaCACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTg                            <  1:198637/140‑1 (MQ=255)                                                                                                    gaCACCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCgg                         >  1:295106/1‑140 (MQ=255)                                                                                                       acCGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCGGTTa                      >  1:313245/1‑140 (MQ=255)                                                                                                         cGCTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCGGTTAtt                    >  1:316993/1‑140 (MQ=255)                                                                                                           cTTAAGGGCGTTGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCGGTTATTGa                  >  2:50081/1‑140 (MQ=255)                                                                                                                     ttGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCGGTTATTGAACGTGAATTg        >  1:341490/1‑140 (MQ=255)                                                                                                                      tGACAGTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCGGTTATTGAACGTGAATTGc       >  1:258815/1‑140 (MQ=255)                                                                                                                           gTCGGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCGGTTATTGAACGTGAATTGCccagc  <  2:121578/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                         AGCGTCACTTCCTCCGTTTGGACGTCAGGGGCAAACCATCGATGTGGTGGTTTCTTCCATGGGAAATGCCAAAAGCTTGCGTGGAGGTACGTTGTTGATGACACCGCTTAAGGGCGTTGACAGTCAGGTGTATGCGCTGGCGCAGGGCAATATTCTGGTTGGCGGCGCAGGAGCCTCCGCTGGCGGTAGCAGTGTTCAGGTTAACCAACTGAACGGTGGACGGATCACCAATGGTGCGGTTATTGAACGTGAATTGCCCAGC  >  NC_000913/1136543‑1136804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑