Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,212,844	A→G	F88F (TTT→TTC)	ycgX ←	uncharacterized protein YcgX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,212,844	0	A	G	100.0%	94.6 / NA	27	F88F (TTT→TTC)	ycgX	uncharacterized protein YcgX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/13);  total (14/13)

TTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGT  >  NC_000913/1212716‑1212980                                                                                                                                 |                                                                                                                                         ttattaTTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGAttt                                                                                                                               >  2:46669/1‑140 (MQ=255)          tCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGc                                                                                                                          >  2:337677/1‑136 (MQ=255)          tCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGc                                                                                                                          <  1:337677/136‑1 (MQ=255)                          ataAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGcc                                                                                                      >  1:184628/1‑140 (MQ=255)                           taAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCct                                                                                                     >  2:134741/1‑140 (MQ=255)                           taAAAGCGTATTATCAATAGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCct                                                                                                     >  2:347687/1‑140 (MQ=255)                              aaGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCtt                                                                                                  >  1:190401/1‑140 (MQ=255)                                   tattatCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGAAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCttttatt                                                                                             >  2:184866/1‑140 (MQ=255)                                    attatCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCttttattt                                                                                            <  1:176754/140‑1 (MQ=255)                                         cAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCa                                                                                       <  1:46669/140‑1 (MQ=255)                                         cAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCa                                                                                       <  2:263936/140‑1 (MQ=255)                                                  agtaACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTg                                                                              >  1:86635/1‑140 (MQ=255)                                                        tGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTa                                                                        >  2:270058/1‑140 (MQ=255)                                                         gCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTAc                                                                       <  2:62603/140‑1 (MQ=255)                                                         gCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTAc                                                                       >  2:333491/1‑140 (MQ=255)                                                          cGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACt                                                                      <  1:270058/140‑1 (MQ=255)                                                            tttGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTaa                                                                    >  2:189050/1‑140 (MQ=255)                                                              tGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACt                                                                  >  1:295741/1‑140 (MQ=255)                                                              tGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACt                                                                  >  1:238350/1‑140 (MQ=255)                                                                ttATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACttt                                                                <  1:290473/140‑1 (MQ=255)                                                                              aGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAggttcctgg                                                  <  2:138726/140‑1 (MQ=255)                                                                               gTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAggttcctggt                                                 >  1:325380/1‑140 (MQ=255)                                                                                             aaCGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACa                                   <  2:66592/140‑1 (MQ=255)                                                                                                    gcgcCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACAAAACCGt                            <  1:134741/140‑1 (MQ=255)                                                                                                     cgcCTGCCAGTGTTGAACAGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACAAAACCGTa                           <  2:184628/140‑1 (MQ=255)                                                                                                                       aGTATTCCCGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAAtt         <  2:295741/140‑1 (MQ=255)                                                                                                                              ccGGAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGt  <  1:333491/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         TTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGTATTCCCGAAATGTAGATTTTCCGCAAATATGAAGCAATGCGGCCTCTTTTATTTTAGCAGGGTTCTTGGTCGTACTAACTTTTAACAGGTTCCTGGTTCCTCTTAATAACAAAACCGTATCATCGTGAGTAATAATTCTGATGT  >  NC_000913/1212716‑1212980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑