Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,225,091	T→C	T91A (ACA→GCA)	minD ←	Z‑ring positioning protein MinD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,225,091	0	T	C	100.0%	56.1 / NA	17	T91A (ACA→GCA)	minD	Z‑ring positioning protein MinD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/8);  total (9/8)

TGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAATCAGGTC  >  NC_000913/1224966‑1225223                                                                                                                              |                                                                                                                                     tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCa                                                                                                                        >  1:56736/1‑140 (MQ=255) tGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCa                                                                                                                        >  1:288039/1‑140 (MQ=255)      ttAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAata                                                                                                                   <  1:346520/140‑1 (MQ=255)              ccGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGAtt                                                                                                           >  1:129492/1‑140 (MQ=255)                ggTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGAtttt                                                                                                         >  1:119220/1‑140 (MQ=255)                   ttCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAg                                                                                                      <  2:288039/140‑1 (MQ=255)                                                                              caAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTGGCGTTGCATCGCCCTGAATGACGTTGACg                                           <  1:335864/140‑1 (MQ=255)                                                                              caAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACg                                           <  2:129492/140‑1 (MQ=255)                                                                               aaGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGa                                          <  2:56736/140‑1 (MQ=255)                                                                                               ccTTCACGGGTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCg                          >  1:174443/1‑140 (MQ=255)                                                                                                       ggTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTcac                  <  2:119220/140‑1 (MQ=255)                                                                                                        gTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCg                          >  1:285351/1‑131 (MQ=255)                                                                                                        gTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCg                          <  2:285351/131‑1 (MQ=255)                                                                                                        gTGAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTcaca                 >  1:331443/1‑140 (MQ=255)                                                                                                          gAGGGCATCTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAAc               <  2:174443/140‑1 (MQ=255)                                                                                                                  cTTTATCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAATc       >  2:220376/1‑140 (MQ=255)                                                                                                                       tCGCGTGCTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAATCAGGTc  >  1:162417/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                     TGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAATGACGTTGACGAAATCGTAAACGACCCGGCGTTCACAACCCATAATCAGGTC  >  NC_000913/1224966‑1225223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑