Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,243,642	C→T	R155W (CGG→TGG)	emtA →	lytic murein transglycosylase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,243,642	0	C	T	100.0%	96.9 / NA	29	R155W (CGG→TGG)	emtA	lytic murein transglycosylase E
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/12);  total (17/12)

TAATATTTCAATGGGGGCGGCTTACCTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTACGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAA  >  NC_000913/1243515‑1243764                                                                                                                                |                                                                                                                           tAATATTTCAATGGGGGCGGCTTACCTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGt                                                                                                                <  1:46643/140‑1 (MQ=255)       ttCAATGGGGGCGGCTTACCTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATc                                                                                                          >  1:22042/1‑140 (MQ=255)         cAATGGGGGCGGCTTACCTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCgg                                                                                                        >  2:202566/1‑140 (MQ=255)                        aCCTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGc                                                                                         >  2:283097/1‑140 (MQ=255)                         ccTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGa                                                                                              <  2:65321/134‑1 (MQ=255)                         ccTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGa                                                                                              >  1:65321/1‑134 (MQ=255)                                    tGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGAt                                                                             >  1:167659/1‑140 (MQ=255)                                           cGGCCCGCTGGCAGGCATTGAAGATCCGATGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAACAAAATCAACGATTTGGa                                                                        <  2:96056/138‑1 (MQ=255)                                           cGGCCCGCTGGCAGGCATTGAAGATCCGATGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAACAAAATCAACGATTTGGa                                                                        >  1:96056/1‑138 (MQ=255)                                                        ggcaTTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCt                                                         <  2:167659/140‑1 (MQ=255)                                                                aGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACg                                                 <  2:22042/140‑1 (MQ=255)                                                                     cGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAgcg                                            <  1:202566/140‑1 (MQ=255)                                                                            aCTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATc                                     >  2:109712/1‑140 (MQ=255)                                                                              tGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACATAGCGCGAAATCAc                                   >  2:116996/1‑140 (MQ=255)                                                                               gCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCAcc                                  >  1:214110/1‑140 (MQ=255)                                                                               gCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACATAGCGCGAAATCAcc                                  <  1:116996/140‑1 (MQ=255)                                                                                 aaTATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCt                                <  2:53501/140‑1 (MQ=255)                                                                                            gtggtgTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGc                     >  1:250849/1‑140 (MQ=255)                                                                                             tggtgTCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCt                    >  2:115209/1‑140 (MQ=255)                                                                                               gtgtCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCt                    <  2:178707/138‑1 (MQ=255)                                                                                               gtgtCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCt                    >  1:178707/1‑138 (MQ=255)                                                                                               gtgtCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTcc                  >  2:273245/1‑140 (MQ=255)                                                                                                tgtCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCcg                 <  1:109712/140‑1 (MQ=255)                                                                                                 gtCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCcgc                >  1:229254/1‑140 (MQ=255)                                                                                                 gtCATACGCTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCcgc                <  2:250849/140‑1 (MQ=255)                                                                                                         cTAACGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATc        >  2:149063/1‑140 (MQ=255)                                                                                                            aCGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTAc     >  1:114568/1‑140 (MQ=255)                                                                                                             cGGGGCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACa    <  2:114568/140‑1 (MQ=255)                                                                                                               gggCAGGTGCGCTGCTATGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACaaa  >  1:225308/1‑140 (MQ=255)                                                                                                                                |                                                                                                                           TAATATTTCAATGGGGGCGGCTTACCTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATATGCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTACGGACTTTCTCGTCAGATCGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTAGCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAA  >  NC_000913/1243515‑1243764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑