Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	141,249	T→C	intergenic (‑24/‑182)	gcd ← / → hpt	quinoprotein glucose dehydrogenase/hypoxanthine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	141,249	0	T	C	100.0%	69.5 / NA	20	intergenic (‑24/‑182)	gcd/hpt	quinoprotein glucose dehydrogenase/hypoxanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

GCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTT  >  NC_000913/141112‑141372                                                                                                                                          |                                                                                                                            gccAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTAGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCaa                                                                                                                           >  1:67721/1‑140 (MQ=255)                       ccGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATtat                                                                                                     <  1:303043/140‑1 (MQ=255)                       ccGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATtat                                                                                                     <  2:63512/140‑1 (MQ=255)                       ccGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATtat                                                                                                     <  1:68723/140‑1 (MQ=255)                                        cAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACAtgtgtg                                                                                    <  1:321324/140‑1 (MQ=255)                                         aGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGa                                                                                   >  2:59720/1‑140 (MQ=255)                                            ccGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCg                                                                                >  1:10686/1‑140 (MQ=255)                                            ccGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCg                                                                                >  2:42303/1‑140 (MQ=255)                                                        gCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTc                                                                    <  1:42303/140‑1 (MQ=255)                                                         cAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCg                                                                   <  2:10686/140‑1 (MQ=255)                                                            aaaGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGc                                                                <  1:104030/140‑1 (MQ=255)                                                                       aGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACA‑GTGTGATCGTCATCACAATTCGa                                                                  >  2:67901/1‑126 (MQ=255)                                                                       aGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACA‑GTGTGATCGTCATCACAATTCGa                                                                  <  1:67901/126‑1 (MQ=255)                                                                                           cgAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGttt                                 <  2:67721/140‑1 (MQ=255)                                                                                           cgAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGttt                                 <  1:10816/140‑1 (MQ=255)                                                                                              gCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTAc                              <  2:3954/140‑1 (MQ=255)                                                                                                        ttAATTGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTg                    >  2:103225/1‑140 (MQ=255)                                                                                                             tGCCATAAAGAGACACCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCgtgt               >  2:292935/1‑140 (MQ=255)                                                                                                                         acacCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTttgt   >  2:20786/1‑140 (MQ=255)                                                                                                                          cacCATTTCAATTCATCAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTttgtt  >  1:59492/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                            GCCAATCGCGACCAGCCAGCCTCCGCCAATGAGTAGATACAGCCCGCAAAGCGCTGCAAAAAGGGCTGTTAGCGTGACGAGTAATCGTCGCGAGCCTGTATTGTTAATTGCCATAAAGAGACACCATTTCAATTCATTAATATTTTAGTAGCAATTAATTATAGGTTTTAACATGTGTGATCGTCATCACAATTCGAGCTTTATTAACAGATTCCGCGAATGAATAGTTTTACTGGTATACTGCGTGTCTTGCGCTTTGTT  >  NC_000913/141112‑141372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑