| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,403,881 | T→C | T229A (ACC→GCC) | abgA ← | p‑aminobenzoyl‑glutamate hydrolase subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,403,881 | 0 | T | C | 91.7% | 28.9 / ‑3.6 | 12 | T229A (ACC→GCC) | abgA | p‑aminobenzoyl‑glutamate hydrolase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (6/5); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01 | |||||||||||
GGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGC > NC_000913/1403765‑1404008 | ggCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCCACAAGGCATTGTGACCGTCTTCTGGGTTTTCGCCTGCGTGAGCGGCGGGGCCGGGGGAGGGGGCGGCAAAATTTGGGGGTGGCC‑TAAAAATATTACtcc > 2:225929/1‑138 (MQ=255) ccgccAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCa > 1:335964/1‑140 (MQ=255) ccgccAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCa < 2:335964/140‑1 (MQ=255) ggCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACgg < 2:197152/140‑1 (MQ=255) tgACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCTGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTaaa < 1:9298/140‑1 (MQ=255) gTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAaataat < 1:324050/140‑1 (MQ=255) ctGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCAAAAAAATATCACTGCCGCACACCCCGGGGCCCGCAGGGACCCCAGTGCCAATGTGCACGGGAGGAAAAAAATCAAca > 2:37899/1‑140 (MQ=255) tGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCCGCACACCACGGGGCCCGCAGGGACGCCAGTGCCAATGTGCACGGCAGTAAAATAAACAACATCATCtc > 2:324050/1‑139 (MQ=255) tGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGGTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGGACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTa > 2:269704/1‑140 (MQ=255) cggcggTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGGACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCg > 2:334227/1‑140 (MQ=255) gcggTACCGGTGAAGTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGAc < 1:183931/140‑1 (MQ=255) gTACCGGGGAAGTGCGCGTC‑AAATTTGGCGGGTGCCATAAAATTATCACCGCCGCACACCACGGGGCCCGCAGGGACGCCCGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCAt > 2:117135/1‑140 (MQ=255) gTGCGCGTC‑AAATTTGGCGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCgcgcgc > 1:48971/1‑140 (MQ=255) cgTC‑AAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCAcgc > 2:242101/1‑140 (MQ=255) | GGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTC‑AAATTTGGTGGTTGCCATAAAATTATCACTGCCGCACACCACGGTGCCCGCAGGTACGCCAGTGCCAATGTGCACGGCAGTAAAATAATCAACATCATCTACGACACCTGCATCGACCATCGCCCGCGCGCCACGC > NC_000913/1403765‑1404008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |