Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,477,669	A→G	T17A (ACG→GCG)	ynbA →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,477,669	0	A	G	100.0%	90.2 / NA	26	T17A (ACG→GCG)	ynbA	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

TCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTAT  >  NC_000913/1477532‑1477792                                                                                                                                          |                                                                                                                            tCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCcggcg                                                                                                                           >  1:208199/1‑140 (MQ=255)           tAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGc                                                                                                                 <  2:208199/140‑1 (MQ=255)                   cGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAg                                                                                                         <  1:256680/140‑1 (MQ=255)                                  tctcCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCg                                                                                          <  2:271785/140‑1 (MQ=255)                                            gggAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAatcacatca                                                                                >  1:4738/1‑140 (MQ=255)                                                    aTGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTtgct                                                                        >  1:310941/1‑140 (MQ=255)                                                         ccATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACt                                                                   >  2:354020/1‑140 (MQ=255)                                                                    aCCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTtctc                                                        >  2:291872/1‑140 (MQ=255)                                                                                       aTAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGtt                                     >  2:68356/1‑140 (MQ=255)                                                                                       aTAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGtt                                     >  1:265969/1‑140 (MQ=255)                                                                                            gACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGt                                >  1:78695/1‑140 (MQ=255)                                                                                             aCGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGAt                                            >  1:227764/1‑127 (MQ=255)                                                                                             aCGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGAt                                            <  2:227764/127‑1 (MQ=255)                                                                                             aCGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGtt                               <  2:78695/140‑1 (MQ=255)                                                                                               gCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATATTgg                             <  2:310941/140‑1 (MQ=255)                                                                                                ctctACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTggc                            >  2:238188/1‑140 (MQ=255)                                                                                                  ctACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTggcgg                          <  1:68356/140‑1 (MQ=255)                                                                                                   tACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTggcggc                         <  2:4738/140‑1 (MQ=255)                                                                                                   tACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTggcggc                         <  2:215525/140‑1 (MQ=255)                                                                                                     ccAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGcac                       <  2:48373/140‑1 (MQ=255)                                                                                                       aGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACaa                     <  1:291872/140‑1 (MQ=255)                                                                                                            aaaCCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCAt                <  2:197222/140‑1 (MQ=255)                                                                                                             aaCCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTggcggc                         <  2:201498/130‑1 (MQ=255)                                                                                                             aaCCGCTCTTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTggcggc                         >  1:201498/1‑130 (MQ=255)                                                                                                                    ctTTCAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCtttt        <  1:238188/140‑1 (MQ=255)                                                                                                                        cAGTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCt    <  1:354020/140‑1 (MQ=255)                                                                                                                          gTCGCTGTTAAGGCCGGCGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTAt  >  1:105991/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                            TCTGAATTTTTAATGAAACGGCGTGTTGCGATATCTCCGTCAGGGGAATTGATGCACCATAGCGCAAACCGAATTATCAAGGATTGATAATGACGCTCTACCAGATAAAACCGCTCTTTCAGTCGCTGTTAAGGCCGACGATGTTTTGGCTTTATAAGCACCACGTTACAGCGAATCACATCACTCTTGCTGCACTGGCGCTTTCTCTTCTTACCGGATTGCTGTTGATGTTGGCGGCACAACCCATCCTCTTTTTGCTAT  >  NC_000913/1477532‑1477792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑