Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,604,249	C→T	T68I (ACC→ATC)	lsrD →	Autoinducer‑2 ABC transporter membrane subunit LsrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,249	0	C	T	100.0%	33.1 / NA	11	T68I (ACC→ATC)	lsrD	Autoinducer‑2 ABC transporter membrane subunit LsrD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/10);  total (1/10)

ATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTA  >  NC_000913/1604119‑1604367                                                                                                                                   |                                                                                                                       aTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTGGTTCGATCATCGGccc                                                                                                               >  2:17579/1‑139 (MQ=255)         gcgAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCca                                                                                                       <  1:234430/140‑1 (MQ=255)                       tcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTa                                                                                         <  1:17579/140‑1 (MQ=255)                       tcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTa                                                                                         <  2:3651/140‑1 (MQ=255)                                    gTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAgtg                                                                            <  1:288255/140‑1 (MQ=255)                                       cAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTTGTTCGATCATCGGCCTCTGCGCCCTTGCATTTGGCGGACTGTTTTTAAgtgggg                                                                         >  2:118692/1‑138 (MQ=255)                                                                   attgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGAc                                             <  1:235065/140‑1 (MQ=255)                                                                      gTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTTGTTTGGTCATCCGCCGCTGCGCCCTTGGATGTGGGGGACTGTTTTAAAGTGGTGGGCCGAGGCCGCTGGCGGTACTCCTGAccct                                          >  2:235065/1‑138 (MQ=255)                                                                            cTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCt                                    <  1:266805/140‑1 (MQ=255)                                                                            cTACCGCTAACGATGGTGATTGTCAGTGGCGGGAACGATATTTCGTTTGGTTTGGTCATCGGGCTCTGCGCCCTTTCATTTGGCGTACTGTTTCAAAGTGGGGTGCCCATGCCGCGGGCGAGAATCCTGACCCTTCTGcc                                    >  2:266805/1‑139 (MQ=255)                                                                                   tAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCa                             <  1:105627/140‑1 (MQ=255)                                                                                       gATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATtgt                         <  1:156262/140‑1 (MQ=255)                                                                                            tGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCggg                    <  1:90497/140‑1 (MQ=255)                                                                                                                          ttGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGatta  >  1:255968/1‑128 (MQ=255)                                                                                                                          ttGGTTCGATCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGatta  <  2:255968/128‑1 (MQ=255)                                                                                                                                   |                                                                                                                       ATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGGCCTCTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTA  >  NC_000913/1604119‑1604367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑