Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,729,457	T→C	V124A (GTC→GCC)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,729,457	0	T	C	100.0%	76.2 / NA	22	V124A (GTC→GCC)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

CACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCG  >  NC_000913/1729343‑1729580                                                                                                                   |                                                                                                                            cACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACt                                                                                                    <  2:174740/140‑1 (MQ=255)                  tCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTg                                                                                       >  2:99423/1‑136 (MQ=255)                  tCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTg                                                                                       <  1:99423/136‑1 (MQ=255)                   cACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAg                                                                                  >  2:251493/1‑140 (MQ=255)                   cACCGATAAAAGCCCTGGGCACCGACGTTCAGCGC‑ACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAgg                                                                                 >  1:123377/1‑140 (MQ=255)                                  tGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTc                                                                   >  2:258607/1‑140 (MQ=255)                                   gggCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCa                                                                  >  2:287942/1‑140 (MQ=255)                                                  gcgcAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCgg                                                   <  1:287942/140‑1 (MQ=255)                                                       aCTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATAtt                                              <  1:262048/140‑1 (MQ=255)                                                           gCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGa                                          <  2:123377/140‑1 (MQ=255)                                                                    gTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGat                                                 <  1:182416/124‑1 (MQ=255)                                                                    gTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGat                                                 >  2:182416/1‑124 (MQ=255)                                                                    gTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCacac                                 <  2:199143/140‑1 (MQ=255)                                                                        aaGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGa                             <  1:258607/140‑1 (MQ=255)                                                                          ggttATTGCCGATGAACGGCGCCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTGCTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCGCCACGCCCGAAt                           <  2:323686/137‑1 (MQ=255)                                                                              aTTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCAct                       >  1:217051/1‑140 (MQ=255)                                                                                  ccGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCacac                                 <  1:219809/126‑1 (MQ=255)                                                                                  ccGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCacac                                 >  2:219809/1‑126 (MQ=255)                                                                                  ccGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTAt                   <  2:217051/140‑1 (MQ=255)                                                                                  ccGATGAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTAt                   >  2:331852/1‑140 (MQ=255)                                                                                       gAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAAt                           <  2:291927/127‑1 (MQ=255)                                                                                       gAACGGCGGCGGCGCGGCGAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAAt                           >  1:291927/1‑127 (MQ=255)                                                                                                   cgcggcgAAACGGAAGCCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCcg  >  1:104489/1‑140 (MQ=255)                                                                                                                   |                                                                                                                            CACGCATCCTCTATATTTCACCGATAAAAGCCCTGGGCACCGACGTTCAGCGCAACTTGCAGATCCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCG  >  NC_000913/1729343‑1729580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑