Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,731,538	T→C	Y818H (TAT→CAT)	lhr →	putative ATP‑dependent helicase Lhr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,731,538	0	T	C	100.0%	23.5 / NA	8	Y818H (TAT→CAT)	lhr	putative ATP‑dependent helicase Lhr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/5);  total (3/5)

CTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCG  >  NC_000913/1731402‑1731604                                                                                                                                         |                                                                   cTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGACATc                                                                 >  1:228542/1‑140 (MQ=255) cTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGACATc                                                                 >  2:289198/1‑140 (MQ=255)             cgccgcCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGACATCCGGATTTTCCgg                                                     <  2:92958/140‑1 (MQ=255)               ccgccCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGACATCCGGATTTTCCGGTc                                                   <  2:228542/140‑1 (MQ=255)                                             cATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGACATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCaa                     <  2:252709/140‑1 (MQ=255)                                              attattAATGCCGGGGCGCACTCCGGGCCATCGCGCCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGACATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAg                    <  2:240549/140‑1 (MQ=255)                                                                             cGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGACATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCg  <  1:303967/127‑1 (MQ=255)                                                                             cGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGACATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCg  >  2:303967/1‑127 (MQ=255)                                                                                                                                         |                                                                   CTCGGCACTTTTCGCCGCCCGTTTTCGCGAATGCGCCGCGCGGGCATTATTAATGCCGGGGCGCACTCCGGGCCATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCG  >  NC_000913/1731402‑1731604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑