| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,752,947 | G→A | P295L (CCG→CTG) | ydhV ← | putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,752,947 | 0 | G | A | 95.7% | 68.5 / ‑3.8 | 23 | P295L (CCG→CTG) | ydhV | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (13/9); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01 | |||||||||||
AAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGCGCCCCAAAACAGCCCTTTACGTGCTGTCCAGCGTGAC > NC_000913/1752811‑1753081 | aaaGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTc > 1:255387/1‑140 (MQ=255) aGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCt > 1:254410/1‑140 (MQ=255) cTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCa > 1:39503/1‑140 (MQ=255) accaccTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTtat > 1:283790/1‑140 (MQ=255) accaccTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTtat > 2:184340/1‑140 (MQ=255) tgCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGa > 1:336944/1‑140 (MQ=255) gggCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCTGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGc < 2:323027/140‑1 (MQ=255) gggCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGc < 2:283790/140‑1 (MQ=255) gggCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGc < 2:255387/140‑1 (MQ=255) gCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCg < 1:184340/140‑1 (MQ=255) cACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGc > 1:223110/1‑137 (MQ=255) cACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGc < 2:223110/137‑1 (MQ=255) aCGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGAc < 2:48660/140‑1 (MQ=255) gCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCg > 1:143981/1‑140 (MQ=255) gCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCg > 2:163808/1‑140 (MQ=255) cAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTa < 2:164989/140‑1 (MQ=255) tCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCt > 1:342694/1‑140 (MQ=255) gCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCcg > 1:82582/1‑128 (MQ=255) gCTCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCcg < 2:82582/128‑1 (MQ=255) tCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGCGCCCCAAAACAGc < 2:254410/140‑1 (MQ=255) tCATTTTTACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGCGCCCCAAAACAGc < 1:47534/140‑1 (MQ=255) tttACTGTGTATTTCTCTGCCGCCAGTCTTAAGTCAAAAGCGGATTTATAGGTACGAATGCCGGCCGTGTTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGAACCCCTTCAGCCGCGACCCAAAACAGCCAttt < 2:39503/140‑1 (MQ=255) tACTGTGTATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGCGCCCCAAAACAGCCCTTTAc > 2:277790/1‑140 (MQ=255) tgtATTTCTCTGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGCGCCCCAAAACAGCCCTTTACGTGCt > 1:236077/1‑140 (MQ=255) tGCCGCCAGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGCGCCCCAAAACAGCCCTTTACGTGCTGTCCAGCGTg > 2:86953/1‑140 (MQ=255) ccgccaGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGCGCCCCAAAACAGCCCTTTACGTGCTGTCCAGCGTGAc > 1:107533/1‑140 (MQ=255) | AAAGTTTGCTACACAAGTGTTACCACCTGTGCTGGGCACGCCAAACTCTTTCACCCGAGGAATATTCATTTGGGTCATACAACGGATCGGGCAAGAGTGGCAGCCGCTCATTTTTACTGTGTATTTCTCTGCCGCCGGTCCTAAGTCAAAAACGGATTTATAGGTACGAAAGCCGACCGTATTCTGATTGCCTGGCGGAATTTCACCCGTTTCAATCGGACCACCTTCAGCCGCGCCCCAAAACAGCCCTTTACGTGCTGTCCAGCGTGAC > NC_000913/1752811‑1753081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |