Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,868,501	T→C	S532P (TCT→CCT)	yeaG →	protein kinase YeaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,868,501	0	T	C	100.0%	38.6 / NA	12	S532P (TCT→CCT)	yeaG	protein kinase YeaG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/6);  total (6/6)

ATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACGCAATCCGA  >  NC_000913/1868400‑1868624                                                                                                      |                                                                                                                            atGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACcggcg                                                                                       <  2:191564/140‑1 (MQ=255)        aaaCATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACcgg                                                                                         <  1:164575/131‑1 (MQ=255)        aaaCATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACcgg                                                                                         >  2:164575/1‑131 (MQ=255)                                   cgcgGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACgaga                                                     >  1:129590/1‑140 (MQ=255)                                    gcgGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGTCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGAt                                                    >  2:337760/1‑140 (MQ=255)                                         tttCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTa                                               <  2:129590/140‑1 (MQ=255)                                                           ggaGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCa                             >  1:222180/1‑140 (MQ=255)                                                                aTCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCg                        >  1:256615/1‑140 (MQ=255)                                                                         cGGATACCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGc               >  1:313074/1‑140 (MQ=255)                                                                              aCCGGGCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACg          <  2:313074/140‑1 (MQ=255)                                                                                  ggCAGCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACGCAAt      <  1:194959/140‑1 (MQ=255)                                                                                      gCTGTTTGACCGCGAGCCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGTCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACGCAATCCGa  <  1:337760/140‑1 (MQ=255)                                                                                                      |                                                                                                                            ATGGGCAAAACATTTTCGACCGTTATGTTACCTACGCGGATTTCTGGATTCAGGATCAGGAGTATCGCGATCCGGATACCGGGCAGCTGTTTGACCGCGAGTCTCTTAACGCCGAGCTGGAGAAAATCGAGAAACCGGCGGGGATCAGTAATCCAAAAGATTTCCGCAACGAGATTGTTAACTTCGTACTGCGCGCCAGAGCGAATAACAGCGGACGCAATCCGA  >  NC_000913/1868400‑1868624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑