Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,922,957	C→T	A5T (GCG→ACG)	pphA ←	phosphoprotein phosphatase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,922,957	0	C	T	100.0%	88.2 / NA	27	A5T (GCG→ACG)	pphA	phosphoprotein phosphatase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/15);  total (12/15)

AGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAGTGTGTGTTATGGTTGACTATAAAGTCAGCG  >  NC_000913/1922822‑1923075                                                                                                                                        |                                                                                                                       agaTAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGc                                                                                                                    >  2:189745/1‑140 (MQ=255)             ctcGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAat                                                                                                        >  2:205790/1‑140 (MQ=255)                           aaaCGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCcgcg                                                                                          <  2:194516/140‑1 (MQ=255)                             aCGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCt                                                                                        <  2:168285/140‑1 (MQ=255)                                  aaTGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGa                                                                                   <  2:291378/140‑1 (MQ=255)                                  aaTGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGa                                                                                   <  1:189745/140‑1 (MQ=255)                                    tGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATc                                                                                 >  1:225095/1‑140 (MQ=255)                                       cATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTtcctc                                                                                               <  2:258521/123‑1 (MQ=255)                                       cATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTtcctc                                                                                               >  1:258521/1‑123 (MQ=255)                                                                     ccGTGTATATCGCCAGAAAGCCAGATATGTCACCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTg                                                >  2:211264/1‑140 (MQ=255)                                                                        tgtATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGAc                                             >  2:131352/1‑140 (MQ=255)                                                                          tataTCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTc                                           <  2:275538/140‑1 (MQ=255)                                                                          tataTCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTc                                           <  1:205790/140‑1 (MQ=255)                                                                               cGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGc                                                  >  1:72658/1‑128 (MQ=255)                                                                               cGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGc                                                  <  2:72658/128‑1 (MQ=255)                                                                               cGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACa                                      >  1:320917/1‑140 (MQ=255)                                                                                 ccAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGt                                    >  2:154721/1‑140 (MQ=255)                                                                                  cAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTa                                   >  2:183506/1‑140 (MQ=255)                                                                                    gAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTaga                                 <  1:183506/140‑1 (MQ=255)                                                                                    gAAAGCCAGATATGTCACCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTaga                                 <  1:211264/140‑1 (MQ=255)                                                                                       aGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAgtg                              <  1:131352/140‑1 (MQ=255)                                                                                           aGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAgtgtgtg                          <  2:320917/140‑1 (MQ=255)                                                                                           aGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAgtgtgtg                          <  1:154721/140‑1 (MQ=255)                                                                                                       aTTGATGACCCGCAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAGTGTGTGTTATGGTTGACt              >  1:56053/1‑140 (MQ=255)                                                                                                                   cAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAGTGTGTGTTATGGTTGACTATAAAGTCAg    <  1:277250/138‑1 (MQ=255)                                                                                                                   cAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAGTGTGTGTTATGGTTGACTATAAAGTCAg    >  2:277250/1‑138 (MQ=255)                                                                                                                   cAATTCTCTGATAAACGGGCGTAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAGTGTGTGTTATGGTTGACTATAAAGTCAGCg  <  2:351777/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                       AGATAAGTAAATCTCGCCACGGATCAAAACGACAATGCCATAATTTGCGGCGCAACTGCTCAAGACAACCGTGTATATCGCCAGAAAGCCAGATATGTCGCCATTGATGACCCGCAATTCTCTGATAAACGGGCGCAGGCTGTTTCATCAATATTTTCCTCCCGCGCTAAAGATCACATAATCTTAACAAGAATGTTAAAAAACGCTGGACTCAGACAGTAGAGTGTGTGTTATGGTTGACTATAAAGTCAGCG  >  NC_000913/1922822‑1923075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑