Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,010,036	G→A	P72P (CCG→CCA)	yedK →	putative SOS response‑associated peptidase YedK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,010,036	0	G	A	100.0%	39.9 / NA	13	P72P (CCG→CCA)	yedK	putative SOS response‑associated peptidase YedK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

TCCCGAACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACA  >  NC_000913/2009907‑2010161                                                                                                                                  |                                                                                                                              tCCCGAACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACg                                                                                                                     >  2:230401/1‑140 (MQ=255) tCCCGAACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACg                                                                                                                     >  2:7916/1‑140 (MQ=255)    cGAACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACGcc                                                                                                                   >  1:286785/1‑139 (MQ=255)    cGAACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACGcc                                                                                                                   <  2:286785/139‑1 (MQ=255)                         cGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCa                                                                                             >  1:152465/1‑140 (MQ=255)                             gcgcCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCGGGGGATATGCCCCCGTTTGGGGGGATAAACCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCg                                                                                         <  2:222247/140‑1 (MQ=255)                                           aGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGc                                                                           <  1:230401/140‑1 (MQ=255)                                              cctcctGCGCAGTAAACGTGATTAACACCTTCTCCTGGAACCGGTTTTCTGGGGATTTGCCCGCGGATGGTGGAATAACCCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGctct                                                                        <  2:317729/140‑1 (MQ=255)                                                   tGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCGGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGCTCTGGCaa                                                                   <  2:152465/140‑1 (MQ=255)                                                       cAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGctct                                                                        <  1:127865/131‑1 (MQ=255)                                                       cAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGctct                                                                        >  2:127865/1‑131 (MQ=255)                                                                  atgaACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATc                                                    >  1:297340/1‑140 (MQ=255)                                                                                                                    tggGATAAACCGCCACTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACa  >  1:296954/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                              TCCCGAACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAACCGCTCTGGCAACATGGTCGGGCAATCTGTTTTGCCGATGGCTGGTTTGAGTGGAAAAAAGAAGGCGACAAAAAACA  >  NC_000913/2009907‑2010161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑