Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,079,625	T→C	T323A (ACA→GCA)	yeeA ←	putative transporter YeeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,079,625	0	T	C	100.0%	75.9 / NA	22	T323A (ACA→GCA)	yeeA	putative transporter YeeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

TGCTGAATCGAAGCAGCAGCAAGATGATTCTGAAGTTCAGGAATTATTTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGTTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTACAGCGCATGAACAAGGCTCAGCAGTATT  >  NC_000913/2079490‑2079761                                                                                                                                        |                                                                                                                                         tGCTGAATCGAAGCAGCAGCAAGATGATTCTGAAGTTCAGGAATTATTTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTcc                                                                                                                                      >  1:210841/1‑140 (MQ=255)  gCTGAATCGAAGCAGCAGCAAGATGATTCTGAAGTTCAGGAATTATTTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCca                                                                                                                                     >  2:90878/1‑140 (MQ=255)   cTGAATCGAAGCAGCAGCAAGATGATTCTGAAGTTCAGGAATTATTTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCcac                                                                                                                                    <  1:323343/140‑1 (MQ=255)         cGAAGCAGCAGCAAGATGATTCTGAAGTTCAGGAATTATTTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCtt                                                                                                                              <  2:257338/140‑1 (MQ=255)         cGAAGCAGCAGCAAGATGATTATGAAGTTCAGGAATTATTTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCtt                                                                                                                              <  2:81183/140‑1 (MQ=255)                                        ggAATTATTTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGc                                                                                               <  2:210841/140‑1 (MQ=255)                                             tattTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGctc                                                                                          <  1:136234/140‑1 (MQ=255)                                                                       aTTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCg                                                                >  1:109326/1‑140 (MQ=255)                                                                                  aCTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCaaaa                                                     >  1:122561/1‑140 (MQ=255)                                                                                   cTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCaaaaa                                                    >  2:262022/1‑140 (MQ=255)                                                                                   cTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCaaaaa                                                    >  2:84184/1‑140 (MQ=255)                                                                                        gCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGc                                               <  1:90878/140‑1 (MQ=255)                                                                                         cTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCt                                              >  1:200362/1‑140 (MQ=255)                                                                                          tGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTg                                             <  1:84184/140‑1 (MQ=255)                                                                                                  cGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTAc                                     >  1:265796/1‑140 (MQ=255)                                                                                                          tGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTAc                             <  2:122561/140‑1 (MQ=255)                                                                                                          tGTTCAGCCACACATAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTAc                             <  1:262022/140‑1 (MQ=255)                                                                                                                    cacaTAACCATAGATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTACAGCGCATGaa                   >  1:271304/1‑140 (MQ=255)                                                                                                                                gATTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTACAGCGCATGAACAAGGCTcagca       >  2:130996/1‑140 (MQ=255)                                                                                                                                 aTTGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTACAGCGCATGAACAAGGCTcagcag      >  1:16038/1‑140 (MQ=255)                                                                                                                                   tGGTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTACAGCGCATGAACAAGGCTCAGCAGTa    >  1:339707/1‑140 (MQ=255)                                                                                                                                    ggTGCTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTGCGTACAGCGCATGAACAAGGCTCAGCAGTAt   <  1:14887/140‑1 (MQ=255)                                                                                                                                     gtgcTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTAAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTACAGCGCATGAACAAGGCTCAGCAGTAtt  >  2:322749/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TGCTGAATCGAAGCAGCAGCAAGATGATTCTGAAGTTCAGGAATTATTTGCGCAAGGCCCGGCAAATCAGATTCGATAGCAACTCAAGCTGATGCGCCGTTTCCATGTTCAGCCACACATAACCATAGATTGGTGTTTCCACAACCTTCAGGTCATGGTGGTTATTGAGCAACTGACGCAGCTCTTCCACAGCATCGTTCAATTTTTCCGTATTGGCAAAAACCGGCTGCGGATTACCTTCGTACAGCGCATGAACAAGGCTCAGCAGTATT  >  NC_000913/2079490‑2079761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑