| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,257,400 | A→G | intergenic (‑65/+29) | psuT ← / ← psuG | putative pseudouridine transporter/pseudouridine‑5'‑phosphate glycosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,257,400 | 0 | A | G | 90.9% | 27.8 / ‑3.9 | 11 | intergenic (‑65/+29) | psuT/psuG | putative pseudouridine transporter/pseudouridine‑5'‑phosphate glycosidase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (7/3); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAG > NC_000913/2257274‑2257535 | gACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGGCGACGGCtt > 2:104865/1‑140 (MQ=255) gaactgtCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCgaga < 1:227527/134‑1 (MQ=255) tCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGa > 1:318726/1‑140 (MQ=255) ttCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCa > 2:62397/1‑140 (MQ=255) tctcATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCaa < 1:62397/140‑1 (MQ=255) atCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTtcgctcg > 2:266398/1‑140 (MQ=255) aTAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAg < 2:318726/140‑1 (MQ=255) tAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGa > 2:134204/1‑140 (MQ=255) cAGCGCCAGGTTTTGGTATGCTTGATGAGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGAttt < 1:266398/140‑1 (MQ=255) tGCTTGATGAGTGCGGGCGACGGCTTTCTGCCCGTAAAAAATTACCCCGCGAGACGCTGATATTCTTTGGCATTTTCGCTCGCAAGAATGGCGTTGTTGAACACCAGCGGGATGTTGGATTTCAGGCTGTCACCGCCGGt < 2:101906/140‑1 (MQ=255) gaGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAg > 1:101906/1‑140 (MQ=255) gaGTGCGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGGTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAg > 1:327707/1‑140 (MQ=255) | GACAACAGAAATGCTATTGCCAGTAACACCACCATCCCCACAACACTTCTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAG > NC_000913/2257274‑2257535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |