Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,334,980	T→C	L555L (CTA→CTG)	yfaA ←	DUF2138 domain‑containing protein YfaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,334,980	0	T	C	100.0%	23.7 / NA	8	L555L (CTA→CTG)	yfaA	DUF2138 domain‑containing protein YfaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/3);  total (5/3)

AGGCGCGAAACAGCCCGGATTGTTCGACATTCAGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATT  >  NC_000913/2334863‑2335108                                                                                                                      |                                                                                                                                 aGGCGCGAAACAGCCCGGATTGTTCGACATTCAGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGCAGCCACTGCCAGGCGGCACCgg                                                                                                            >  2:153869/1‑140 (MQ=255)   gcgcGAAACAGCCCGGATTGTTCGACATTCAGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGCAGCCACTGCCAGGCGGCACCGGGt                                                                                                          >  2:323036/1‑140 (MQ=255)                                              gggCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCAGTGCCTCATAGCGGCTGCCAGGTTATGGGCAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATa                                                               <  2:328950/140‑1 (MQ=255)                                                          aacacaGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGCAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCt                                                   >  2:306026/1‑140 (MQ=255)                                                               agccagcAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGCAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGc                                              >  1:81690/1‑140 (MQ=255)                                                               agccagcAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGCAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGc                                              >  1:84664/1‑140 (MQ=255)                                                                                                 tAGCGGCTGCCAGGTTATGGGCAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCg            <  1:153869/140‑1 (MQ=255)                                                                                                           cAGGTTATGGGCAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGGGAtt  <  1:323036/140‑1 (MQ=255)                                                                                                                      |                                                                                                                                 AGGCGCGAAACAGCCCGGATTGTTCGACATTCAGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATT  >  NC_000913/2334863‑2335108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑