Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,656,884	T→C	E67G (GAG→GGG)	fdx ←	reduced ferredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,656,884	0	T	C	100.0%	99.9 / NA	28	E67G (GAG→GGG)	fdx	reduced ferredoxin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/15);  total (13/15)

TAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGATACCGTTACGCAGAGCTGCGTCGAGAATGGTTTCACCGC  >  NC_000913/2656771‑2657016                                                                                                                  |                                                                                                                                     tAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCaaaa                                                                                                            >  2:81123/1‑140 (MQ=255)   gtgtAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAAcc                                                                                                          >  2:78009/1‑140 (MQ=255)     gtAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCaaa                                                                                                             <  2:126768/135‑1 (MQ=255)     gtAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCaaa                                                                                                             >  1:126768/1‑135 (MQ=255)     gtAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCtt                                                                                                        >  2:6308/1‑140 (MQ=255)     gtAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCtt                                                                                                        >  2:338924/1‑140 (MQ=255)     gtAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCtt                                                                                                        >  2:223905/1‑140 (MQ=255)         cgcgGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacg                                                                                                    >  1:273052/1‑140 (MQ=255)          gcgGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacga                                                                                                   >  2:119585/1‑140 (MQ=255)           cgGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaa                                                                                                  <  2:327920/140‑1 (MQ=255)            gggATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCacgaac                                                                                                 <  1:150422/140‑1 (MQ=255)                tttCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATg                                                                                             <  1:223905/140‑1 (MQ=255)                tttCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATg                                                                                             <  1:119585/140‑1 (MQ=255)                  tCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCa                                                                                           <  1:159470/140‑1 (MQ=255)                      ctactaAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTgg                                                                                       <  1:81123/140‑1 (MQ=255)                       tactaAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGc                                                                                      <  1:110200/140‑1 (MQ=255)                        actaAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCa                                                                                     >  2:280546/1‑140 (MQ=255)                         ctaAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAg                                                                                    <  2:199509/140‑1 (MQ=255)                         ctaAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAg                                                                                    <  2:27145/140‑1 (MQ=255)                                     cgGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGca                                                                        <  1:78009/140‑1 (MQ=255)                                                  cTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTcac                                                           >  2:353484/1‑140 (MQ=255)                                                              aCGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCaa                                               <  2:33458/140‑1 (MQ=255)                                                                                       gCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGATACCGTTACGCagag                          >  2:38419/1‑136 (MQ=255)                                                                                       gCTTTGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGATACCGTTACGCagag                          <  1:38419/136‑1 (MQ=255)                                                                                          ttGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGATACCGTTACg                               >  2:231257/1‑128 (MQ=255)                                                                                          ttGTCCAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGATACCGTTACg                               <  1:231257/128‑1 (MQ=255)                                                                                              ccAGCATGTCGTCTTCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGATACCGTTACGCAGAGCTGCGTCgaga               <  1:6308/140‑1 (MQ=255)                                                                                                           ttCCTGCCCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGATACCGTTACGCAGAGCTGCGTCGAGAATGGTTTCACCGc  >  1:252125/1‑140 (MQ=255)                                                                                                                  |                                                                                                                                     TAGTGTAACGCGGGATTTCGACTACTAAATCTTCGTCGGTAACGCGCGCCTGGCAGCTTAAACGGCTTTCCGGCTCCAGTCCCCAGGCTTTGTCCAGCATGTCGTCTTCCTGCTCTGAGCTTTCCGGCAGTGAGTCAAAACCTTCACGAACGATGCAGTGGCAGGTGGTGCAAGCACAGGATTTTTCACAGGCGTGTTCAATCTCGATACCGTTACGCAGAGCTGCGTCGAGAATGGTTTCACCGC  >  NC_000913/2656771‑2657016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑