Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,671,549	T→C	F109L (TTC→CTC)	hcaB →	2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,671,549	0	T	C	100.0%	63.7 / NA	19	F109L (TTC→CTC)	hcaB	2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/10);  total (9/10)

ATCAGATCCTGACTCGTTCCGGCAAGCTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCTTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGTCAAATGCCGCCTGGTATCCTGGCGGCGGTGGC  >  NC_000913/2671439‑2671683                                                                                                               |                                                                                                                                       aTCAGATCCTGACTCGTTCCGGCAAGCTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTtctc                                                                                                           >  2:332215/1‑140 (MQ=255)        ccTGACTCGTTCCGGCAAGCTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTGCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTAcc                                                                                                    >  2:317869/1‑140 (MQ=255)        ccTGACTCGTTCCGGCAAGCTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTAcc                                                                                                    >  1:158816/1‑140 (MQ=255)            aCTCGTTCCGGCAAGCTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACctgct                                                                                                >  1:217756/1‑140 (MQ=255)            aCTCGTTCCGGCAAGCTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACctgct                                                                                                <  1:332215/140‑1 (MQ=255)                gTTCCGGCAAGCTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGgc                                                                                            >  2:291366/1‑140 (MQ=255)                                            cAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCg                                                                <  1:291366/140‑1 (MQ=255)                                                   ggCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGa                                                         >  1:224738/1‑140 (MQ=255)                                                          gggATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGgcagc                                                  <  1:230860/140‑1 (MQ=255)                                                              tCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGa                                              >  1:344789/1‑140 (MQ=255)                                                                    tGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTca                                        <  2:158816/140‑1 (MQ=255)                                                                      ccTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTcaca                                      <  2:344789/140‑1 (MQ=255)                                                                          aCTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGt                                  >  1:55682/1‑140 (MQ=255)                                                                              gTTAATACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGTCaaa                              >  1:349671/1‑140 (MQ=255)                                                                                 aaTACTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGTCAAATgc                           <  2:349671/140‑1 (MQ=255)                                                                                     cTCCCGCAGAGACGCTCGAAACCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGTCAAATgccgcc                       <  1:215143/140‑1 (MQ=255)                                                                                                        aaCCGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGTCAAATGCCGCCTGGTATCCTGGCGGCGGTg    <  2:55682/140‑1 (MQ=255)                                                                                                          ccGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGTCAAATGCCGCCTGGTATCCTGGCGGCGGTGGc  <  2:224738/140‑1 (MQ=255)                                                                                                          ccGGCCTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGTCAAATGCCGCCTGGTATCCTGGCGGCGGTGGc  <  2:217756/140‑1 (MQ=255)                                                                                                               |                                                                                                                                       ATCAGATCCTGACTCGTTCCGGCAAGCTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAACCGGCTTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCAGTGAAGGCAGCATGATTTTCACACTGTCAAATGCCGCCTGGTATCCTGGCGGCGGTGGC  >  NC_000913/2671439‑2671683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑