Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,776,823	T→C	V146V (GTT→GTC)	yfjY →	CP4‑57 prophage; RadC‑like JAB domain‑containing protein YfjY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,776,823	0	T	C	100.0%	64.2 / NA	19	V146V (GTT→GTC)	yfjY	CP4‑57 prophage; RadC‑like JAB domain‑containing protein YfjY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/7);  total (12/7)

TGCAGCAGCAGTGATACTGGCGCATAACCACCCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTTGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAGAATTATCAGTAAACGCCGGGCAA  >  NC_000913/2776688‑2776904                                                                                                                                        |                                                                                  tGCAGCAGCAGTGATACTGGCGCATAACCACCCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTcggtg                                                                               >  2:120582/1‑140 (MQ=255)      cagcagTGATACTGGCGCATAACCACCCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTggcagg                                                                          <  1:147085/140‑1 (MQ=255)                   ggCGCATAACCACCCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCACTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTAttcgt                                                             >  2:168194/1‑140 (MQ=255)                       cATAACCACCCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGc                                                         <  2:114160/140‑1 (MQ=255)                        aTAACCACCCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCa                                                        >  2:165081/1‑140 (MQ=255)                         tAACCACCCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAg                                                       >  2:275109/1‑140 (MQ=255)                           accaccCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGaa                                                     >  1:164781/1‑140 (MQ=255)                           accaccCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGaa                                                     <  2:164781/140‑1 (MQ=255)                                cccTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACgg                                                >  1:9835/1‑140 (MQ=255)                                 ccTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGt                                               <  1:165081/140‑1 (MQ=255)                                     cGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGc                                           >  1:138266/1‑140 (MQ=255)                                                     agccagGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATg                           >  2:123748/1‑140 (MQ=255)                                                      gccagGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATga                          >  2:78769/1‑140 (MQ=255)                                                                      tataACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACg          <  2:138266/140‑1 (MQ=255)                                                                      tataACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACg          >  2:177467/1‑140 (MQ=255)                                                                      tataACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACg          <  2:63964/140‑1 (MQ=255)                                                                         aaCGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACGcag       >  1:246051/1‑138 (MQ=255)                                                                             cAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACGCAGGGCa   >  2:106871/1‑140 (MQ=255)                                                                              aGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACGCAGGGCaa  >  2:157621/1‑140 (MQ=255)                                                                              aGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACGCAGGGCaa  <  1:78769/140‑1 (MQ=255)                                                                                                                                        |                                                                                  TGCAGCAGCAGTGATACTGGCGCATAACCACCCCTCCGGCGACACTACTCCCAGCCAGGCAGATAAGACTATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTTGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAGAATTATCAGTAAACGCCGGGCAA  >  NC_000913/2776688‑2776904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑