Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,776,881	G→A	R2K (AGA→AAA)	ypjJ →	CP4‑57 prophage; DUF987 domain‑containing protein YpjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,776,881	0	G	A	100.0%	18.6 / NA	7	R2K (AGA→AAA)	ypjJ	CP4‑57 prophage; DUF987 domain‑containing protein YpjJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

TATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTTGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAGAATTATCAGTAAACGCCGGGCAA  >  NC_000913/2776757‑2776904                                                                                                                             |                        tataACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACg          <  2:138266/140‑1 (MQ=255) tataACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACg          >  2:177467/1‑140 (MQ=255) tataACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACg          <  2:63964/140‑1 (MQ=255)    aaCGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACGcag       >  1:246051/1‑138 (MQ=255)        cAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACGCAGGGCa   >  2:106871/1‑140 (MQ=255)         aGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACGCAGGGCaa  <  1:78769/140‑1 (MQ=255)         aGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTCGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAAAATTATCAGTAAACGCAGGGCaa  >  2:157621/1‑140 (MQ=255)                                                                                                                             |                        TATAACGCAGCGTCTGGTGCAGGCGCTTCAGCTCGTTGATATCCGTGTCCCTGACCATCTGATTGTTGGTGGCAGGCAAATCTATTCGTTCGCAGAACACGGTCTGCTTTGAGGTATTACATGAGAATTATCAGTAAACGCCGGGCAA  >  NC_000913/2776757‑2776904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑