Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,776,957	A→C	G27G (GGA→GGC)	ypjJ →	CP4‑57 prophage; DUF987 domain‑containing protein YpjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,776,957	0	A	C	100.0%	41.5 / NA	13	G27G (GGA→GGC)	ypjJ	CP4‑57 prophage; DUF987 domain‑containing protein YpjJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/8);  total (5/8)

CAATGACGATATACCGCCAGCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGAAAATATCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGATGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCGGACCGTATGCTTGACTGATGAGTATCAC  >  NC_000913/2776902‑2777087                                                        |                                                                                                                                   cAATGACGATATACCGCCAGCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGaa                                                <  2:246051/140‑1 (MQ=255)  aaTGACGATATACCGCCAGCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAAc                                               <  2:64560/140‑1 (MQ=255)               cGCCAGCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGc                                  >  2:245797/1‑140 (MQ=255)                gCCAGCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCa                                 >  1:172010/1‑140 (MQ=255)                 ccAGCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAg                                >  1:159736/1‑140 (MQ=255)                 ccAGCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAg                                >  2:145520/1‑140 (MQ=255)                    gCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCgg                             >  2:252455/1‑140 (MQ=255)                        ccTGAGTCCCGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCGGACCg                         <  1:106871/140‑1 (MQ=255)                                 cGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCGGACCGTATGCTTGa                <  1:145520/140‑1 (MQ=255)                                 cGAATCTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCGGACCGTATGCTTGa                <  1:252455/140‑1 (MQ=255)                                      cTTTCGCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCGGACCGTATGCTTGACtgat           <  1:134844/140‑1 (MQ=255)                                           gCTACTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCGGACCGTATGCTTGACTGATGAGTa      <  1:323060/140‑1 (MQ=255)                                               cTGCACCGGCAAATACCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGACGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCGGACCGTATGCTTGACTGATGAGTATCAc  <  1:76215/140‑1 (MQ=255)                                                        |                                                                                                                                   CAATGACGATATACCGCCAGCATCCTGAGTCCCGAATCTTTCGCTACTGCACCGGAAAATATCAGTGGCACGGTAGCGTCTGTCATTACACCGGCAGGGATGTTCCGGATATCACAGGAGTCCTGGCTGTGTACGCCGAACGCCGCAGGACCGCAGCGGACCGTATGCTTGACTGATGAGTATCAC  >  NC_000913/2776902‑2777087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑