Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	285,648	C→G	A85G (GCC→GGC)	yagG →	putative D‑xylonate transporter YagG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	285,648	0	C	G	100.0%	90.0 / NA	26	A85G (GCC→GGC)	yagG	putative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (9/17);  total (9/17)

TGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCC  >  NC_000913/285513‑285784                                                                                                                                         |                                                                                                                                         tGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATc                                                                                                                                      <  1:21273/140‑1 (MQ=255)                     aCGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGt                                                                                                                  >  2:106059/1‑140 (MQ=255)                                   ctcCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTaca                                                                                                    <  1:90692/140‑1 (MQ=255)                                     cccGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACg                                                                                                  >  1:267287/1‑140 (MQ=255)                                         cgTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCg                                                                                               <  1:286386/140‑1 (MQ=255)                                          gTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                                                                                             <  2:267287/140‑1 (MQ=255)                                          gTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                                                                                             <  2:212034/140‑1 (MQ=255)                                          gTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                                                                                             <  1:241077/140‑1 (MQ=255)                                          gTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                                                                                             <  2:154318/140‑1 (MQ=255)                                                gACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                                                                                             >  1:207366/1‑134 (MQ=255)                                                gACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa                                                                                             <  2:207366/134‑1 (MQ=255)                                                    ccGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGca                                                                                   <  1:164464/140‑1 (MQ=255)                                                            gACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCaa                                                                           <  1:194614/140‑1 (MQ=255)                                                            gACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTAACCTTCTACACGCCGGACTTCTCCGCACAGGGCaa                                                                           >  1:114834/1‑140 (MQ=255)                                                              cccGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGa                                                                         <  2:101813/140‑1 (MQ=255)                                                                     aTGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTCCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTa                                                                  >  1:110130/1‑140 (MQ=255)                                                                         ggCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGcc                                                              >  2:107365/1‑140 (MQ=255)                                                                           ctgctgGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTg                                                            >  2:81028/1‑140 (MQ=255)                                                                            tgctgGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGc                                                           <  1:81028/140‑1 (MQ=255)                                                                                 gTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGAc                                                      <  1:107365/140‑1 (MQ=255)                                                                                              gcacGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTg                                         >  1:271616/1‑140 (MQ=255)                                                                                                 cgcgGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTAACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGAcc                                      <  2:114834/140‑1 (MQ=255)                                                                                                  gcgGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTCCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGAccc                                     <  2:110130/140‑1 (MQ=255)                                                                                                   cgGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGAcc                                      <  2:168876/138‑1 (MQ=255)                                                                                                   cgGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGAcc                                      >  1:168876/1‑138 (MQ=255)                                                                                                                    cccGTTCCTGCTGT‑GGGGGGGCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTa                   <  2:271616/140‑1 (MQ=255)                                                                                                                                    ggggggCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTgcg    >  2:136045/1‑140 (MQ=255)                                                                                                                                    ggggggCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTgcg    <  1:265757/140‑1 (MQ=255)                                                                                                                                      ggggCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGcc  <  1:96297/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGT‑GGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTTCTCCGCACAGGGCAAGATCATCTACGCCTGCGTGACCTACATTCTCCTGACCCTGGTCTACACCTTCGTTAACGTGCCGTACTGCGCC  >  NC_000913/285513‑285784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑