Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,903,312	T→C	Y473H (TAT→CAT)	ygcE →	putative sugar kinase YgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,903,312	0	T	C	100.0%	76.1 / NA	22	Y473H (TAT→CAT)	ygcE	putative sugar kinase YgcE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/12);  total (10/12)

CTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAAGGCCGGGCGGGAAACTGCCCGGCCTGAACATACCTGAATGGTTATCCCCGCTGACGCGGGGAAC  >  NC_000913/2903181‑2903446                                                                                                                                    |                                                                                                                                       cTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTg                                                                                                                                <  2:99171/140‑1 (MQ=255)              ggTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCAtggggaat                                                                                                                   >  1:140559/1‑140 (MQ=255)                    cgTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCAtggggaattgggga                                                                                                             >  2:216829/1‑140 (MQ=255)                             tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGAtt                                                                                                    <  1:101624/140‑1 (MQ=255)                             tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGAtt                                                                                                    <  2:266655/140‑1 (MQ=255)                             tatCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGAtt                                                                                                    <  1:326593/140‑1 (MQ=255)                                     taCCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCag                                                                                            >  2:274509/1‑140 (MQ=255)                                         gATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCt                                                                                        >  1:53495/1‑140 (MQ=255)                                            tGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGt                                                                                     >  2:303930/1‑140 (MQ=255)                                             gagaGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTc                                                                                    <  2:139809/140‑1 (MQ=255)                                                    aTGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCg                                                                             <  2:53495/140‑1 (MQ=255)                                                     tGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGt                                                                            >  2:228933/1‑140 (MQ=255)                                                     tGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGt                                                                            >  1:206061/1‑140 (MQ=255)                                                       ccAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTaa                                                                          <  2:173129/140‑1 (MQ=255)                                                       ccAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTaa                                                                          <  2:206061/140‑1 (MQ=255)                                                               tACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGaa                                                                  >  1:215089/1‑140 (MQ=255)                                                                                 ggCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAAGGCCGGGCGGGAAACTGc                                                <  1:228933/140‑1 (MQ=255)                                                                                      tttCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAAGGCCGGGCGGGAAACTGCCCGGc                                           <  1:303930/140‑1 (MQ=255)                                                                                          aaaGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAAGGCCGGGCGGGAAACTGCCCGGCCTGa                                       <  1:274509/140‑1 (MQ=255)                                                                                                  aaCCAAACATACTGATGTGATCCTGAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAAGGCCGGGCGGGAAACTGCCCGGCCTGAACATACCt                               >  2:21512/1‑140 (MQ=255)                                                                                                                          gAAAAAATCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAAGGCCGGGCGGGAAACTGCCCGGCCTGAACATACCTGAATGGTTATCCCCGCTGACGCgg       >  1:29365/1‑140 (MQ=255)                                                                                                                               aaTCGCATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAAGGCCGGGCGGGAAACTGCCCGGCCTGAACATACCTGAATGGTTATCCCCGCTGACGCGGGGAAc  <  1:21512/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       CTGTCGATAACATGGTTCGCGTGAAAGATATCTTTATACCGATTGAGAGCAATGCCAAACGCTACGACGCGATGAATAAAGGCATTTTCAAAGACCTAACCAAACATACTGATGTGATCCTGAAAAAATCGTATGAAGTGATGCATGGGGAATTGGGGAATGTGGATTCGATCCAGAGCTGGTCGAATGCGTAAGTGAGGAAGGCCGGGCGGGAAACTGCCCGGCCTGAACATACCTGAATGGTTATCCCCGCTGACGCGGGGAAC  >  NC_000913/2903181‑2903446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑