| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,909,358 | T→C | E103E (GAA→GAG) | pyrG ← | CTP synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,909,358 | 0 | T | C | 100.0% | 37.2 / NA | 12 | E103E (GAA→GAG) | pyrG | CTP synthetase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (6/6); total (6/6) | |||||||||||
TACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTC > NC_000913/2909237‑2909492 | tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAg < 1:341754/133‑1 (MQ=255)tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAg > 2:341754/1‑133 (MQ=255)tACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCa > 2:187751/1‑140 (MQ=255) cgccgATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCagag < 1:187751/140‑1 (MQ=255) tGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGa < 1:262830/133‑1 (MQ=255) tGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGa > 2:262830/1‑133 (MQ=255) cGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGcggcgg > 1:188639/1‑140 (MQ=255) gCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTa < 2:222520/140‑1 (MQ=255) aaTAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAg < 1:211204/124‑1 (MQ=255) aaTAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAg > 2:211204/1‑124 (MQ=255) gTTGCGCCGAGGTAGTCACCGCGGCGCTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGcc < 1:314368/140‑1 (MQ=255) cggcgCTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTc > 2:278275/1‑140 (MQ=255) | TACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTC > NC_000913/2909237‑2909492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |