Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,945,671	T→C	T58A (ACG→GCG)	tcdA ←	tRNA threonylcarbamoyladenosine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,945,671	0	T	C	100.0%	75.7 / NA	22	T58A (ACG→GCG)	tcdA	tRNA threonylcarbamoyladenosine dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/7);  total (15/7)

TTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAA  >  NC_000913/2945536‑2945805                                                                                                                                        |                                                                                                                                       ttACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGAtt                                                                                                                                    >  2:318461/1‑140 (MQ=255)         cACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAAt                                                                                                                            >  2:135184/1‑140 (MQ=255)           cTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATcc                                                                                                                          >  2:128377/1‑140 (MQ=255)               gggTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGt                                                                                                                      <  1:128377/140‑1 (MQ=255)                              atccGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTc                                                                                                       <  1:71798/137‑1 (MQ=255)                                                tCCGCTTTTGCCAGCCCAACGTTATCGCTCAGGGCATGAGTTTGCCGATTGGTATTGGTGACGCACACACCATCCATATCGATAAGCGCGATTGCGCCAGTCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGac                                                                                     <  2:152627/140‑1 (MQ=255)                                                tCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGac                                                                                     <  1:295448/140‑1 (MQ=255)                                                      tttGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACaccacc                                                                               >  2:307457/1‑140 (MQ=255)                                                                           cgcAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATg                                                          <  2:300236/140‑1 (MQ=255)                                                                                       aTTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCaaa                                              >  1:157825/1‑140 (MQ=255)                                                                                        tttGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAAc                                             >  1:59012/1‑140 (MQ=255)                                                                                                        ggtGACGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCttttt                             <  1:135184/140‑1 (MQ=255)                                                                                                             cGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCa                        >  1:94886/1‑140 (MQ=255)                                                                                                             cGCACACATCATCCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCa                        >  2:234988/1‑140 (MQ=255)                                                                                                                       atcCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGAcgcg              <  2:94886/140‑1 (MQ=255)                                                                                                                        tcCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACgcgc             >  1:87778/1‑140 (MQ=255)                                                                                                                        tcCATATCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACgcgc             >  2:159488/1‑140 (MQ=255)                                                                                                                              tCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGcc       >  1:157117/1‑140 (MQ=255)                                                                                                                              tCGATAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGcc       >  2:332777/1‑140 (MQ=255)                                                                                                                                 aTAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGccacc    >  1:316635/1‑140 (MQ=255)                                                                                                                                  tAAGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGccacca   >  2:23502/1‑140 (MQ=255)                                                                                                                                   aaGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCaa  <  1:307457/140‑1 (MQ=255)                                                                                                                                   aaGCGCGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCaa  >  1:262805/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TTACACGGCACTCCGGGTTAATCTGGCGAATACGCTCCGCCATAACTTCCGCTTTTGCCAGCCCAACGTTATCGCGCAGGGCATGAATTTGCCGATTGGTATTGGTGACGCACACATCATCCATATCGATAAGCGTGATTGCGCCAATCCCCGTGCGCGCCAGCGCTTCCGCCGCCCAGGAACCGACACCACCGATACCAACCACACAAATATGCGCGTCAGCAAACAGTTGCAACGCTTTTTCACCATACAGACGCGCTGTGCCACCAA  >  NC_000913/2945536‑2945805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑