Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,965,981	T→C	G19G (GGA→GGG)	lgt ←	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,965,981	0	T	C	100.0%	34.7 / NA	11	G19G (GGA→GGG)	lgt	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/5);  total (6/5)

TAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGTCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCA  >  NC_000913/2965858‑2966114                                                                                                                            |                                                                                                                                      tAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGa                                                                                                                       >  2:292352/1‑140 (MQ=255)   aGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGAcc                                                                                                                     >  2:137721/1‑140 (MQ=255)                       gTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATg                                                                                                 <  1:244565/140‑1 (MQ=255)                                cTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCt                                                                                        <  1:267455/140‑1 (MQ=255)                                             tCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGt                                                                           >  2:212372/1‑140 (MQ=255)                                                  cgtcgtGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCCCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTcacca                                                                      <  2:308786/140‑1 (MQ=255)                                                                                                    gCCGTACCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGcgccg                    >  1:318437/1‑140 (MQ=255)                                                                                                      cGTACCGGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGAtt                                 >  1:354421/1‑125 (MQ=255)                                                                                                      cGTACCGGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGAtt                                 <  2:354421/125‑1 (MQ=255)                                                                                                         aCCAGTGAAGCGCCACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGt               >  1:329463/1‑140 (MQ=255)                                                                                                                      cACGGGCCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCa  <  2:94171/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                      TAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATTGCAAAAATGAAACCCACCAGATACATCAGGCCGTACCAGTGAAGCGCCACGGGTCCTATTGAGAAAATGACCGGATCAAACTCCGGAAAATGCAGATAGCTACTGGTCATCTGTCACCACAAGTTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCA  >  NC_000913/2965858‑2966114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑