Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,006,504	G→A	A81A (GCG→GCA)	ygeW →	putative carbamoyltransferase YgeW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,006,504	0	G	A	100.0%	55.2 / NA	17	A81A (GCG→GCA)	ygeW	putative carbamoyltransferase YgeW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/4);  total (13/4)

CGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTATTGGTATTCGCGACGATATGTAT  >  NC_000913/3006381‑3006639                                                                                                                            |                                                                                                                                        cGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGcc                                                                                                                         <  2:94767/140‑1 (MQ=255) cGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGcc                                                                                                                         >  1:94767/1‑140 (MQ=255)    tGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTg                                                                                                                      >  1:321281/1‑140 (MQ=255)                      aCTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTcgacg                                                                                                    >  2:42331/1‑140 (MQ=255)                           gTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGc                                                                                               <  1:42331/140‑1 (MQ=255)                             gCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCaa                                                                                             <  2:284368/140‑1 (MQ=255)                                  aaaCATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATcac                                                                                        >  1:126632/1‑140 (MQ=255)                                        ctcAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCg                                                                                  <  2:125081/140‑1 (MQ=255)                                                 aGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCg                                                                         >  1:88735/1‑140 (MQ=255)                                                                    ggTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGc                                                      >  1:323513/1‑140 (MQ=255)                                                                         ttCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAata                                                 >  2:241334/1‑140 (MQ=255)                                                                                     cgACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCt                                     >  1:338386/1‑140 (MQ=255)                                                                                     cgACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCt                                     >  1:306265/1‑140 (MQ=255)                                                                                                  cGTACCCGCTTCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTAt                        >  1:270547/1‑140 (MQ=255)                                                                                                           ttCTCTTATGCTTCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTATTGGTATTcg               >  1:197299/1‑140 (MQ=255)                                                                                                               tttATGCTTCCGCACTTACCCTGCTCGCCCTTCCACAAAAAGATCTCGACGAAGGAAAATCACAAACCGCCCACGGCAAAACCGTGCGTAAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTATTGGTATTCGcgac           <  2:209663/139‑1 (MQ=255)                                                                                                                       tCCGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTATTGGTATTCGCGACGATATGTa   >  1:209663/1‑140 (MQ=255)                                                                                                                        ccGCACTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTATTGGTATTCGCGACGATATGTAt  >  2:46331/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                        CGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTATTGGTATTCGCGACGATATGTAT  >  NC_000913/3006381‑3006639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑