Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,298,668	G→A	T60I (ACC→ATC)	yraR ←	putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,298,668	0	G	A	100.0%	102.4 / NA	30	T60I (ACC→ATC)	yraR	putative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/15);  total (15/15)

TATGCTGCGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGAC  >  NC_000913/3298531‑3298803                                                                                                                                          |                                                                                                                                        tatGCTGCGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATg                                                                                                                                       >  1:131066/1‑140 (MQ=255)                     cgccCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACt                                                                                                                   <  1:81624/140‑1 (MQ=255)                      gccCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTc                                                                                                                  <  2:131066/140‑1 (MQ=255)                           gTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACACCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTg                                                                                                             >  2:149012/1‑140 (MQ=255)                             tAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACACCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCg                                                                                                           <  1:149012/140‑1 (MQ=255)                                 gCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTg                                                                                                             >  2:177427/1‑134 (MQ=255)                                 gCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTg                                                                                                             <  1:177427/134‑1 (MQ=255)                                   ggTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCAt                                                                                                     <  1:176866/140‑1 (MQ=255)                                    gTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATg                                                                                                    >  1:29291/1‑140 (MQ=255)                                           cTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGc                                                                                                            >  2:91386/1‑125 (MQ=255)                                           cTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGc                                                                                                            <  1:91386/125‑1 (MQ=255)                                            tACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTaa                                                                                            <  1:155476/140‑1 (MQ=255)                                                 gCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACAcc                                                                                       >  1:72417/1‑140 (MQ=255)                                                    tgtAATCGGCATGAACAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAacac                                                                                        <  2:34237/136‑1 (MQ=255)                                                    tgtAATCGGCATGAACAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAacac                                                                                        >  1:34237/1‑136 (MQ=255)                                                          cGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCata                                                                              <  1:135810/140‑1 (MQ=255)                                                            gCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACAcccc                                                                                     <  1:187165/131‑1 (MQ=255)                                                            gCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACAcccc                                                                                     >  2:187165/1‑131 (MQ=255)                                                              aTGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGc                                                                          <  1:142892/140‑1 (MQ=255)                                                                           tctTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGAcgt                                                               <  1:33114/138‑1 (MQ=255)                                                                           tctTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGAcgt                                                               >  2:33114/1‑138 (MQ=255)                                                                                              gccgcGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCg                                          >  1:173640/1‑140 (MQ=255)                                                                                                                 acaaAACACAATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTaa                       >  2:213955/1‑140 (MQ=255)                                                                                                                       cacaATGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACa                 <  1:213955/140‑1 (MQ=255)                                                                                                                            tGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCgc            >  1:245993/1‑140 (MQ=255)                                                                                                                            tGTCGATGGGATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCgc            >  1:274112/1‑140 (MQ=255)                                                                                                                                    ggATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCg             <  2:180439/131‑1 (MQ=255)                                                                                                                                    ggATCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCg             >  1:180439/1‑131 (MQ=255)                                                                                                                                      aTCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGAc  <  2:245993/140‑1 (MQ=255)                                                                                                                                      aTCGATGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGAc  >  1:122038/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TATGCTGCGCGCCCAGTCGCCGCCCGGTTAATGCGGTATCCACTACCAGCGTGTAATCGGCATGAATAAACGCCTCTTTGCTCCCCGCTTCTCGCCGCGTGGTGCCGAGACAACAAAACACAATGTCGATGGGATCGGTGACCTGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGAC  >  NC_000913/3298531‑3298803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑