Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,860,630	(C)5→4	coding (348/894 nt)	yidL →	putative DNA‑binding transcriptional regulator YidL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,860,626	0	C	.	100.0%	240.0 / NA	55	coding (344/894 nt)	yidL	putative DNA‑binding transcriptional regulator YidL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (30/25);  total (30/25)

CAAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTACCCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCG  >  NC_000913/3860487‑3860760                                                                                                                                            |                                                                                                                                       cAAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAc                                                                                                                                        <  1:1119517/140‑1 (MQ=255) cAAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAc                                                                                                                                        <  2:1117298/140‑1 (MQ=255)  aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGTAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       >  1:1013718/1‑140 (MQ=255)  aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       >  2:755999/1‑140 (MQ=255)  aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       >  2:698651/1‑140 (MQ=255)  aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       >  2:586135/1‑140 (MQ=255)  aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       <  1:827624/140‑1 (MQ=255)  aaGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcc                                                                                                                                       >  1:743889/1‑140 (MQ=255)    ggATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcccc                                                                                                                                     >  2:1042091/1‑140 (MQ=255)    ggATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcccc                                                                                                                                     >  1:251336/1‑140 (MQ=255)     gATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAcccca                                                                                                                                    <  2:1133554/140‑2 (MQ=255)      aTGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGTAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccac                                                                                                                                   <  2:1013718/140‑3 (MQ=255)      aTGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccac                                                                                                                                   >  2:905955/1‑138 (MQ=255)      aTGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccac                                                                                                                                   <  1:905955/140‑3 (MQ=255)        gtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccacca                                                                                                                                 <  1:891637/140‑5 (MQ=255)        gtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccacca                                                                                                                                 <  1:755999/140‑5 (MQ=255)        gtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccacca                                                                                                                                 <  1:399291/140‑5 (MQ=255)        gtgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTAccccacca                                                                                                                                 <  1:183992/140‑5 (MQ=255)         tgGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAg                                                                                                                               >  1:666226/1‑140 (MQ=255)           ggAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTa                                                                                                                             <  1:698651/140‑1 (MQ=255)             aaaaTTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTatg                                                                                                                           <  2:15303/140‑1 (MQ=255)                     tttAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGatat                                                                                                                   <  1:713696/140‑1 (MQ=255)                         aaGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTccc                                                                                                               >  2:1051908/1‑140 (MQ=255)                          aGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCg                                                                                                              <  1:1051908/140‑1 (MQ=255)                               ggCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGt                                                                                                         <  1:1093028/140‑1 (MQ=255)                                      aaaTAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCaaa                                                                                                  >  2:799465/1‑140 (MQ=255)                                         tAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCg                                                                                               <  2:227709/140‑1 (MQ=255)                                         tAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCg                                                                                               <  2:261576/140‑1 (MQ=255)                                                 cATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGttatttat                                                                                       >  1:434263/1‑140 (MQ=255)                                                  aTGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTtata                                                                                      >  2:338638/1‑140 (MQ=255)                                                  aTGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTtata                                                                                      >  2:430310/1‑140 (MQ=255)                                                    ggCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAAt                                                                                    >  1:6848/1‑140 (MQ=255)                                                      cAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATgg                                                                                  >  2:465045/1‑140 (MQ=255)                                                      cAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATgg                                                                                  >  1:109946/1‑140 (MQ=255)                                                       aaCTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGTTATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGc                                                                                 >  2:301408/1‑140 (MQ=255)                                                        aCTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGAACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCg                                                                                <  1:185360/140‑1 (MQ=255)                                                         cTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGa                                                                               <  2:251336/140‑1 (MQ=255)                                                         cTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGa                                                                               <  1:799465/140‑1 (MQ=255)                                                         cTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGa                                                                               >  1:703187/1‑140 (MQ=255)                                                            tattatATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAAt                                                                            >  1:310998/1‑140 (MQ=255)                                                             attatATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAAtt                                                                           <  1:969238/140‑1 (MQ=255)                                                               tataTTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTta                                                                         <  1:430310/140‑1 (MQ=255)                                                               tataTTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTta                                                                         <  1:1011370/140‑1 (MQ=255)                                                                    tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    >  1:993959/1‑140 (MQ=255)                                                                    tCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATc                                                                    >  1:766556/1‑140 (MQ=255)                                                                        aaaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGa                                                                >  2:670294/1‑140 (MQ=255)                                                                        aaaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGa                                                                <  1:586135/140‑1 (MQ=255)                                                                         aaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGaa                                                               <  2:620462/140‑1 (MQ=255)                                                                         aaGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGaa                                                               <  2:6848/140‑1 (MQ=255)                                                                           gCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAgg                                                                 >  2:681686/1‑136 (MQ=255)                                                                           gCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAgg                                                                 <  1:681686/136‑1 (MQ=255)                                                                           gCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACt                                                             >  1:618122/1‑140 (MQ=255)                                                                            ccAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTc                                                            <  2:703864/140‑1 (MQ=255)                                                                                tGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCg                                                        >  1:944945/1‑140 (MQ=255)                                                                                tGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCg                                                        >  1:475892/1‑140 (MQ=255)                                                                                      ttCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTg                                                  <  1:670294/140‑1 (MQ=255)                                                                                      ttCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTg                                                  <  2:766556/140‑1 (MQ=255)                                                                                       tCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGc                                                 <  1:482752/140‑1 (MQ=255)                                                                                        cACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                <  1:465045/140‑1 (MQ=255)                                                                                               aTCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                <  1:605355/133‑1 (MQ=255)                                                                                               aTCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCt                                                >  2:605355/1‑133 (MQ=255)                                                                                                  aCTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCTCGTTGGTCAGCAACGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTtaataa                                      >  2:404904/1‑140 (MQ=255)                                                                                                     gtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACtt                                   <  2:475892/140‑1 (MQ=255)                                                                                                     gtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACtt                                   >  2:632734/1‑140 (MQ=255)                                                                                                     gtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACtt                                   >  1:226470/1‑140 (MQ=255)                                                                                                     gtgAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACtt                                   >  1:148491/1‑140 (MQ=255)                                                                                                              tAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          >  2:343783/1‑140 (MQ=255)                                                                                                              tAGTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAg                          <  1:343783/140‑1 (MQ=255)                                                                                                                gTCTGGGAACAGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCa                        >  1:817600/1‑140 (MQ=255)                                                                                                                          aGTACTGGATGGAATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAaata              >  2:1110016/1‑140 (MQ=255)                                                                                                                                 gatggaATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGc       >  2:731253/1‑140 (MQ=255)                                                                                                                                 gatggaATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGc       >  2:875760/1‑140 (MQ=255)                                                                                                                                    ggaATTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGt    >  1:51310/1‑140 (MQ=255)                                                                                                                                      aaTTTA‑CCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCg  >  2:357188/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       CAAGGATGTGGGAAAATTCTTTTAAAGAATGGCGAACAAATAACACTGCATGGCAACTGTATTATATTCTTAAAGCCAATGGATATTCACTCTTATCACTGTGAAGGTTTAGTCTGGGAACAGTACTGGATGGAATTTACCCCCACCAGTATGATGGATATTCCCGTTGGTCAGCAAAGCGTTATTTATAATGGCGAAATTTATAATCAGGAACTCACCGAAGTTGCTGAGTTAATAACTTCACCAGAAGCAATAAAAAATAATCTGGCAGTCG  >  NC_000913/3860487‑3860760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑