Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,120,438	(C)6→7	coding (1117/1395 nt)	wcaJ ←	undecaprenyl‑phosphate glucose phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,120,432	1	.	C	100.0%	217.9 / NA	60	M375V (ATG→GTG)	wcaJ	undecaprenyl‑phosphate glucose phosphotransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (34/26);  total (34/26)

GCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACAT‑CCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCA  >  NC_000913/2120298‑2120560                                                                                                                                        |                                                                                                                                  gccgcGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACAT‑ccccc                                                                                                                              <  1:723330/140‑1 (MQ=255)      gcCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACAT‑cccccccggt                                                                                                                         <  2:148951/140‑5 (MQ=255)         aGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAg                                                                                                                       <  1:311060/140‑1 (MQ=255)           ccGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGca                                                                                                                     <  2:266039/140‑1 (MQ=255)           ccGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGca                                                                                                                     <  1:624143/140‑1 (MQ=255)           ccGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGca                                                                                                                     <  2:117021/140‑1 (MQ=255)           ccGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGca                                                                                                                     <  1:83949/140‑1 (MQ=255)                      cgcCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAAc                                                                                                          >  2:967917/1‑140 (MQ=255)                      cgcCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAAc                                                                                                          >  1:554599/1‑140 (MQ=255)                      cgcCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAAc                                                                                                          >  1:373288/1‑140 (MQ=255)                       gcCCAGCCGGTAATGCCCGGTTTCTCCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGCCATCCCCCCCGGTCAg                                                                                                                       <  1:1076468/126‑1 (MQ=255)                       gcCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGTACCGACAATCGACATCCCCCCCGGTCAg                                                                                                                       >  2:1076468/1‑126 (MQ=255)                        cccAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTg                                                                                                        >  2:30074/1‑140 (MQ=255)                         ccAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGc                                                                                                       <  1:1027399/140‑1 (MQ=255)                          cAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCg                                                                                                      <  1:967917/140‑1 (MQ=255)                          cAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGGCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCg                                                                                                      <  1:290703/140‑1 (MQ=255)                           aGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCgg                                                                                                     >  2:873115/1‑140 (MQ=255)                            gCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGc                                                                                                    <  2:729034/140‑1 (MQ=255)                                       ccGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAg                                                                                         >  2:1005866/1‑140 (MQ=255)                                       ccGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAg                                                                                         >  1:913530/1‑140 (MQ=255)                                               aCCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTAc                                                                                 <  1:30074/140‑1 (MQ=255)                                                ccTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACg                                                                                >  1:122976/1‑140 (MQ=255)                                                    aTGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGgcgc                                                                            >  1:522057/1‑140 (MQ=255)                                                    aTGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGgcgc                                                                            >  2:568882/1‑140 (MQ=255)                                                     tGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCa                                                                           >  1:183646/1‑140 (MQ=255)                                                      ggCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACg                                                                                <  2:1057329/134‑1 (MQ=255)                                                      ggCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACg                                                                                >  1:1057329/1‑134 (MQ=255)                                                          cagcaTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAg                                                                      >  1:449633/1‑140 (MQ=255)                                                             caTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTc                                                                   >  1:541845/1‑140 (MQ=255)                                                             caTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTc                                                                   >  1:838690/1‑140 (MQ=255)                                                               tGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTccc                                                                 <  1:568882/140‑1 (MQ=255)                                                                 tAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCa                                                               >  1:96436/1‑140 (MQ=255)                                                                       tCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTgg                                                         >  1:208494/1‑140 (MQ=255)                                                                        cAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGt                                                        <  1:340245/140‑1 (MQ=255)                                                                         aaTGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTg                                                       >  1:1015680/1‑140 (MQ=255)                                                                             aGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGAcgc                                                   >  2:643050/1‑140 (MQ=255)                                                                               cTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTccc                                                                 <  2:1038382/124‑1 (MQ=255)                                                                               cTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTccc                                                                 >  1:1038382/1‑124 (MQ=255)                                                                                  tCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGa                                              >  1:766077/1‑140 (MQ=255)                                                                                  tCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGa                                              <  2:449633/140‑1 (MQ=255)                                                                                    gATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATc                                            >  2:858694/1‑140 (MQ=255)                                                                                          gTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTg                                      <  2:838690/140‑1 (MQ=255)                                                                                            tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGGGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑Cg                                   <  2:373288/140‑1 (MQ=255)                                                                                            tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑Cg                                   >  1:636747/1‑140 (MQ=255)                                                                                            tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑Cg                                   >  2:81475/1‑140 (MQ=255)                                                                                                tATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGc                               >  2:359170/1‑140 (MQ=255)                                                                                                 aTGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGcc                              <  2:290035/140‑1 (MQ=255)                                                                                                  tGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCt                             <  2:913530/140‑1 (MQ=255)                                                                                                        aCTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTca                       <  2:208494/140‑1 (MQ=255)                                                                                                            cgtgcgGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTcaccac                   >  1:19170/1‑140 (MQ=255)                                                                                                            cgtgcgGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTaaccac                   >  2:40976/1‑140 (MQ=255)                                                                                                              tgcgGACGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACtt                 >  1:617467/1‑140 (MQ=255)                                                                                                                   aCGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCg            <  1:1005866/140‑1 (MQ=255)                                                                                                                   aCGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCg            >  1:596005/1‑140 (MQ=255)                                                                                                                   aCGTGGACCGACAATCGACATCCCCCCCGGCCACCCCATGGTTAAACTGGGGCAATTCATCCAGCGAGGAACGGCCCAAAAAGTTCCCCACTTTGGGAACCCCCGGACCG‑TCTGCCGCCGCCGGGGCCACAACttggccg            >  2:135402/1‑135 (MQ=255)                                                                                                                    cGTGGACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCGt           <  1:873115/140‑1 (MQ=255)                                                                                                                        gACCGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTAGTtctc       >  2:717996/1‑140 (MQ=255)                                                                                                                           cGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCGTTCTCCAt    <  1:359170/140‑1 (MQ=255)                                                                                                                           cGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCGTTCTCCAt    <  1:81475/140‑1 (MQ=255)                                                                                                                           cGACAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCGTTCTCCAt    <  1:858694/140‑1 (MQ=255)                                                                                                                             aCAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCa  >  2:695686/1‑140 (MQ=255)                                                                                                                             aCAATCGACATCCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCa  >  1:78344/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  GCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACAT‑CCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTG‑CGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCA  >  NC_000913/2120298‑2120560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑