Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	567	A→G	G77G (GGA→GGG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	567	0	A	G	100.0%	201.4 / NA	54	G77G (GGA→GGG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

GTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCG  >  NC_000913/433‑699                                                                                                                                       |                                                                                                                                     gTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTcgc                                                                                                                                 <  1:153648/140‑1 (MQ=255) gTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTcgc                                                                                                                                 >  1:389446/1‑140 (MQ=255)         cGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGccc                                                                                                                         >  2:503600/1‑140 (MQ=255)           tCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTcgccgccg                                                                                                                            <  1:1013583/135‑1 (MQ=255)           tCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTcgccgccg                                                                                                                            >  2:1013583/1‑135 (MQ=255)            ccTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGTTGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGc                                                                                                                      <  1:364445/140‑1 (MQ=255)            ccTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGc                                                                                                                      <  1:805942/140‑1 (MQ=255)            ccTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGc                                                                                                                      <  1:1135773/140‑1 (MQ=255)              tctctGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCg                                                                                                                    <  2:515331/140‑1 (MQ=255)              tctctGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCg                                                                                                                    >  1:46699/1‑140 (MQ=255)                tctGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCggg                                                                                                                  >  2:1045763/1‑140 (MQ=255)                      cccGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTccc                                                                                                            >  2:592803/1‑140 (MQ=255)                        cGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCa                                                                                                                        <  2:441265/126‑1 (MQ=255)                        cGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCa                                                                                                                        >  1:441265/1‑126 (MQ=255)                        cGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGc                                                                                                          >  1:960914/1‑140 (MQ=255)                         gCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCt                                                                                                         >  1:447470/1‑140 (MQ=255)                             aaaTCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGgcg                                                                                                     <  2:960914/140‑1 (MQ=255)                                          ccTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACtt                                                                                        >  1:127960/1‑140 (MQ=255)                                                  cGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGAt                                                                                >  1:567667/1‑140 (MQ=255)                                                    atgatTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTtcg                                                                                     >  2:1076204/1‑133 (MQ=255)                                                    atgatTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTtcg                                                                                     <  1:1076204/133‑1 (MQ=255)                                                    atgatTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCa                                                                              <  2:567667/140‑1 (MQ=255)                                                     tgatTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCG‑ACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTtcg                                                                                     >  2:71671/1‑131 (MQ=255)                                                     tgatTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCG‑ACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTtcg                                                                                     <  1:71671/131‑1 (MQ=255)                                                         tGAAAAAACCATTAGCGGCCAGGATGCTTTACACAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAAt                                                                         >  2:486776/1‑140 (MQ=255)                                                                aCCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCa                                                                  >  2:407298/1‑140 (MQ=255)                                                                     tAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATaa                                                             >  2:670274/1‑140 (MQ=255)                                                                       gCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATaaaa                                                           <  1:1045763/140‑1 (MQ=255)                                                                           ccAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATg                                                       <  1:592803/140‑1 (MQ=255)                                                                               gATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCt                                                   >  1:186026/1‑140 (MQ=255)                                                                                   cTTTACACAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCAt                                               <  1:486776/140‑1 (MQ=255)                                                                                     ttACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATgg                                             <  2:447470/140‑1 (MQ=255)                                                                                      tACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGc                                            >  2:776974/1‑140 (MQ=255)                                                                                         ccAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATg                                              >  2:964177/1‑135 (MQ=255)                                                                                         ccAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATg                                              <  1:964177/135‑1 (MQ=255)                                                                                         ccAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCAtt                                         >  1:163577/1‑140 (MQ=255)                                                                                           aaTATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAg                                       <  2:127960/140‑1 (MQ=255)                                                                                             tatCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGc                                                 <  1:523431/128‑1 (MQ=255)                                                                                             tatCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGc                                                 >  2:523431/1‑128 (MQ=255)                                                                                                cAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTttgt                                  >  2:187922/1‑140 (MQ=255)                                                                                                 aGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTttgtt                                 >  1:310588/1‑140 (MQ=255)                                                                                                  gCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTttgttg                                >  2:930990/1‑140 (MQ=255)                                                                                                  gCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTttgttg                                >  2:643011/1‑140 (MQ=255)                                                                                                      tGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGc                            <  1:670274/140‑1 (MQ=255)                                                                                                      tGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGc                            <  2:163577/140‑1 (MQ=255)                                                                                                      tGCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGc                            >  2:38257/1‑140 (MQ=255)                                                                                                       gCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCa                           >  1:231872/1‑140 (MQ=255)                                                                                                       gCCGAACGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCa                           <  2:231872/140‑1 (MQ=255)                                                                                                            aCGTATTTTTGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGcc                      <  2:186026/140‑1 (MQ=255)                                                                                                                 tttttGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGAt                 <  1:776974/140‑1 (MQ=255)                                                                                                                 tttttGCCGAACTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGAt                 <  1:38257/140‑1 (MQ=255)                                                                                                                          aaCTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCAt            <  1:922690/136‑1 (MQ=255)                                                                                                                          aaCTTTTGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCAt            >  2:922690/1‑136 (MQ=255)                                                                                                                                tGACGGGGCTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAAcgctgcg  <  1:503600/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     GTGGCCACCGTCCTCTCTGCCCCCGCCAAAATCACCAACCACCTGGTGGCGATGATTGAAAAAACCATTAGCGGCCAGGATGCTTTACCCAATATCAGCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCG  >  NC_000913/433‑699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑