Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 429,923 C→T A140V (GCA→GTA)  secF → Sec translocon accessory complex subunit SecF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,9230CT100.0% 134.0 / NA 43A140V (GCA→GTA) secFSec translocon accessory complex subunit SecF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/19);  total (24/19)

GGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCT  >  NC_000913/429789‑430058
                                                                                                                                      |                                                                                                                                       
ggTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGAcc                                                                                                                                    <  2:554832/140‑1 (MQ=255)
   ccGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTg                                                                                                                                 >  2:1068749/1‑140 (MQ=255)
              ctgctgAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGt                                                                                                                      >  2:324209/1‑140 (MQ=255)
                gctgAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGc                                                                                                                    >  1:155960/1‑140 (MQ=255)
                gctgAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGc                                                                                                                    >  2:192070/1‑140 (MQ=255)
                gctgAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGc                                                                                                                    >  1:864388/1‑140 (MQ=255)
                      ggCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGc                                                                                                              >  1:1018462/1‑140 (MQ=255)
                           aaCCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGa                                                                                                         >  1:524150/1‑140 (MQ=255)
                                 cggTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                   <  2:462840/140‑1 (MQ=255)
                                   gTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcg                                                                                                 <  2:524150/140‑1 (MQ=255)
                                     cAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAG‑TCGTCGGTCCGAGCGTGGGGGTAGCCCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                              <  2:178731/140‑1 (MQ=255)
                                     cAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAG‑TCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                              <  2:700431/140‑1 (MQ=255)
                                      aGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                              <  2:155960/140‑1 (MQ=255)
                                        gtgtTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctgct                                                                                            <  1:192070/140‑1 (MQ=255)
                                             gggCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTa                                                                                       <  2:862445/140‑1 (MQ=255)
                                                cAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATcc                                                                                    <  1:1068749/140‑1 (MQ=255)
                                                  gCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                  >  1:526536/1‑140 (MQ=255)
                                                                gTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCATATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCGGCGCTGCTGTCTATCCTCGTGTACGTAGGttt                                                                    <  2:528690/140‑1 (MQ=255)
                                                                  gATTAACGAATCCACCAATCAGAATGCAGCAGTGTAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTcc                                                                  >  2:511448/1‑140 (MQ=255)
                                                                  gATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTcc                                                                  >  2:635287/1‑140 (MQ=255)
                                                                  gATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTcc                                                                  >  2:809184/1‑140 (MQ=255)
                                                                  gATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTcc                                                                  >  2:1116266/1‑140 (MQ=255)
                                                                  gATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTcc                                                                  >  1:31973/1‑140 (MQ=255)
                                                                  gATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTcc                                                                  >  1:721334/1‑140 (MQ=255)
                                                                       aCGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCttt                                                             <  1:1116266/140‑1 (MQ=255)
                                                                       aCGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCttt                                                             <  1:809184/140‑1 (MQ=255)
                                                                          aaTCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACTGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAg                                                          >  1:531075/1‑140 (MQ=255)
                                                                          aaTCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAg                                                          >  1:44666/1‑140 (MQ=255)
                                                                           aTCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGt                                                         >  2:914345/1‑140 (MQ=255)
                                                                                             agcagTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAgggg                                       >  1:978544/1‑140 (MQ=255)
                                                                                                  tGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGtt                                  >  1:58747/1‑140 (MQ=255)
                                                                                                              aGTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGgcg                      <  1:914345/140‑1 (MQ=255)
                                                                                                               gTTCGTCGGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGgcgc                     <  2:526536/140‑1 (MQ=255)
                                                                                                                 tcgtcgGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGGGGTTATTGCGCTGGCGCac                   >  2:168643/1‑140 (MQ=255)
                                                                                                                  cgtcgGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCacg                  <  2:58747/140‑1 (MQ=255)
                                                                                                                   gtcgGTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCacga                 >  1:195220/1‑140 (MQ=255)
                                                                                                                      ggTCCTAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt              <  1:519013/140‑1 (MQ=255)
                                                                                                                      ggTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt              <  2:111753/140‑1 (MQ=255)
                                                                                                                      ggTCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt              <  2:1018462/140‑1 (MQ=255)
                                                                                                                        tCCGAGCGTGGGGGTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTa            >  1:912850/1‑140 (MQ=255)
                                                                                                                                 gggggTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGc   <  2:44666/140‑1 (MQ=255)
                                                                                                                                  ggggTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt  >  2:76744/1‑140 (MQ=255)
                                                                                                                                  ggggTAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt  >  2:809830/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                       
GGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCT  >  NC_000913/429789‑430058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑