Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,445,549	A→G	T480A (ACC→GCC)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,445,549	0	A	G	100.0%	321.5 / NA	86	T480A (ACC→GCC)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/51);  total (35/51)

GTTGCTCGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAA  >  NC_000913/4445413‑4445685                                                                                                                                         |                                                                                                                                         gTTGCTCGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCt                                                                                                                                       >  2:920723/1‑140 (MQ=255)    gCTCGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGc                                                                                                                                    >  2:80590/1‑140 (MQ=255)               aaCAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTg                                                                                                                         >  2:484225/1‑140 (MQ=255)                 cAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGcc                                                                                                                       <  1:764589/140‑1 (MQ=255)                   ggCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCgg                                                                                                                     >  1:640418/1‑140 (MQ=255)                    gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGa                                                                                                                    >  1:218091/1‑140 (MQ=255)                    gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGa                                                                                                                    >  1:307607/1‑140 (MQ=255)                        ttAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTg                                                                                                                >  1:20045/1‑140 (MQ=255)                         tAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGa                                                                                                               <  1:1103552/140‑1 (MQ=255)                           gTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAg                                                                                                             <  1:77721/140‑1 (MQ=255)                           gTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCACCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAg                                                                                                             <  2:965238/140‑1 (MQ=255)                             tGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCt                                                                                                           <  1:230453/140‑1 (MQ=255)                              ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                          >  2:556934/1‑140 (MQ=255)                              ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                          >  1:441561/1‑140 (MQ=255)                                cgcgGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGAc                                                                                                        >  2:838819/1‑140 (MQ=255)                                   ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTGGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  2:1063457/140‑1 (MQ=255)                                   ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGTACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  2:215553/140‑1 (MQ=255)                                   ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     >  1:34528/1‑140 (MQ=255)                                   ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  2:34528/140‑1 (MQ=255)                                    gTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGt                                                                                                    <  2:194396/140‑1 (MQ=255)                                    gTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGt                                                                                                    >  2:738581/1‑140 (MQ=255)                                      gAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTaa                                                                                                  >  1:1048908/1‑140 (MQ=255)                                        gCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACg                                                                                                <  1:546241/140‑1 (MQ=255)                                          tAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGtt                                                                                              <  2:1048908/140‑1 (MQ=255)                                               cTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACa                                                                                         >  2:1107230/1‑140 (MQ=255)                                               cTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACa                                                                                         >  1:170241/1‑140 (MQ=255)                                                 tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGa                                                                                       <  2:1142578/140‑1 (MQ=255)                                                    cGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACa                                                                                    <  2:88204/140‑1 (MQ=255)                                                     ggCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACaa                                                                                   <  2:441561/140‑1 (MQ=255)                                                      gCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACaaa                                                                                  <  2:656139/140‑1 (MQ=255)                                                        aTTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGt                                                                                <  1:990480/140‑1 (MQ=255)                                                        aTTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGt                                                                                >  2:1033454/1‑140 (MQ=255)                                                        aTTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGt                                                                                >  1:83034/1‑140 (MQ=255)                                                          tAACACCGCTAAAGAGTTCCCGGACTGGCCGGCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTAGGGCGGCGCCTGGCAGATCGTCGTGCCGGCGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGa                                                                              <  2:221429/140‑1 (MQ=255)                                                           aaCACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGaa                                                                             <  2:218091/140‑1 (MQ=255)                                                              acCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGt                                                                          <  2:20045/140‑1 (MQ=255)                                                                cGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTg                                                                        >  1:88040/1‑140 (MQ=255)                                                                 gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGa                                                                       >  2:232642/1‑140 (MQ=255)                                                                  ccAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGAc                                                                      >  2:44981/1‑140 (MQ=255)                                                                  ccAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGAc                                                                      >  2:726585/1‑140 (MQ=255)                                                                  ccAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGAc                                                                      >  1:294555/1‑140 (MQ=255)                                                                   cAAAGAGTTCCCGTACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACg                                                                     <  2:204856/140‑1 (MQ=255)                                                                        agTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACg                                                                <  2:893966/140‑1 (MQ=255)                                                                         gTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGc                                                               <  2:25243/140‑1 (MQ=255)                                                                               ggACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGc                                                               <  1:324142/134‑1 (MQ=255)                                                                               ggACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGc                                                               >  2:324142/1‑134 (MQ=255)                                                                                 aCTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       <  1:738581/140‑1 (MQ=255)                                                                                 aCTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       <  1:438400/140‑1 (MQ=255)                                                                                 aCTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       <  1:1033454/140‑1 (MQ=255)                                                                                 aCTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       <  1:717474/140‑1 (MQ=255)                                                                                 aCTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       <  1:896791/140‑1 (MQ=255)                                                                                   tGGCCGTCGAAACTCAATGGCTTGATTAAAGCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  2:82305/140‑1 (MQ=255)                                                                                   tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  2:640418/140‑1 (MQ=255)                                                                                   tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:556934/140‑1 (MQ=255)                                                                                   tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:1107230/140‑1 (MQ=255)                                                                                    ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAAc                                                    >  2:757016/1‑140 (MQ=255)                                                                                    ggCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAAc                                                    >  1:924282/1‑140 (MQ=255)                                                                                     gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                                   >  2:543803/1‑140 (MQ=255)                                                                                     gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACa                                                   <  1:893872/140‑1 (MQ=255)                                                                                          cGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTtat                                              <  2:307607/140‑1 (MQ=255)                                                                                              aCTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGc                                          <  1:920723/140‑1 (MQ=255)                                                                                                   aTGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTgg                                     <  2:806031/140‑1 (MQ=255)                                                                                                    tGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGga                                    >  2:221579/1‑140 (MQ=255)                                                                                                    tGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGga                                    >  2:383219/1‑140 (MQ=255)                                                                                                     ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  1:838819/140‑1 (MQ=255)                                                                                                     ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  1:543803/140‑1 (MQ=255)                                                                                                     ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  1:1081099/140‑1 (MQ=255)                                                                                                     ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  1:398323/140‑1 (MQ=255)                                                                                                          gATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCa                                         <  2:987166/129‑1 (MQ=255)                                                                                                          gATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCa                                         >  1:987166/1‑129 (MQ=255)                                                                                                           aTTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATccc                             <  1:44981/140‑1 (MQ=255)                                                                                                            ttAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   >  2:406527/1‑133 (MQ=255)                                                                                                            ttAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  1:406527/133‑1 (MQ=255)                                                                                                              aaaaCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAgg                          <  2:579463/140‑1 (MQ=255)                                                                                                                aaCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGc                        <  2:924282/140‑1 (MQ=255)                                                                                                                aaCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGc                        >  2:1081908/1‑140 (MQ=255)                                                                                                                 aCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACATTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCt                       <  1:315752/140‑1 (MQ=255)                                                                                                                     gcgGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCAt                   >  1:539063/1‑140 (MQ=255)                                                                                                                            ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  2:88040/140‑1 (MQ=255)                                                                                                                            ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  1:726585/140‑1 (MQ=255)                                                                                                                              tGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTc          <  2:690822/140‑1 (MQ=255)                                                                                                                                tACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCgg        >  1:419444/1‑140 (MQ=255)                                                                                                                                 aCGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCggg       <  1:221579/140‑1 (MQ=255)                                                                                                                                     cggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaa   >  1:1114205/1‑140 (MQ=255)                                                                                                                                     cggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaa   >  1:545786/1‑140 (MQ=255)                                                                                                                                      ggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaaa  <  1:232642/140‑1 (MQ=255)                                                                                                                                      ggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaaa  <  1:484225/140‑1 (MQ=255)                                                                                                                                      ggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaaa  <  1:80590/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTTGCTCGACTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAA  >  NC_000913/4445413‑4445685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑