Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,507,190	T→C	intergenic (+81/‑7)	yjhE → / → insO	KpLE2 phage‑like element; putative membrane protein (pseudogene)/KpLE2 phage‑like element; IS911B regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,507,190	0	T	C	100.0%	304.5 / NA	81	intergenic (+81/‑7)	yjhE/insO	KpLE2 phage‑like element; putative membrane protein (pseudogene)/KpLE2 phage‑like element; IS911B regulator fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/29);  total (52/29)

TTGCTTGTTATTGGTATATTCCTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCA  >  NC_000913/4507053‑4507326                                                                                                                                          |                                                                                                                                         ttGCTTGTTATTGGTATATTCCTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGgcgc                                                                                                                                        >  1:436451/1‑140 (MQ=255)          aTTGGTATATTCCTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGaa                                                                                                                               <  2:5508/140‑1 (MQ=255)            tGGTATATTCCTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGaaaa                                                                                                                             >  1:382703/1‑140 (MQ=255)              gTATATTCCTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGaaaaaa                                                                                                                           >  2:504436/1‑140 (MQ=255)              gTATATTCCTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGaaaaaa                                                                                                                           >  2:1138757/1‑140 (MQ=255)                  atTCCTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGaa                                                                                                                       >  1:913363/1‑140 (MQ=255)                     ccTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAAtt                                                                                                                    >  2:926919/1‑140 (MQ=255)                       tGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTc                                                                                                                  <  2:382703/140‑1 (MQ=255)                                       tCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAAc                                                                                                  >  1:423736/1‑140 (MQ=255)                                         cAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAAcgc                                                                                                >  2:930852/1‑140 (MQ=255)                                         cAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAAcgc                                                                                                >  2:531017/1‑140 (MQ=255)                                         cAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAAcgc                                                                                                >  2:631979/1‑140 (MQ=255)                                         cAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAAcgc                                                                                                >  2:603601/1‑140 (MQ=255)                                           gATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGa                                                                                              <  1:631979/140‑1 (MQ=255)                                            aTAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGaa                                                                                             <  1:930852/140‑1 (MQ=255)                                                gTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCg                                                                                         >  2:878010/1‑140 (MQ=255)                                                gTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCg                                                                                         >  1:804105/1‑140 (MQ=255)                                                gTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCg                                                                                         >  1:991601/1‑140 (MQ=255)                                                 tCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGc                                                                                        >  2:89186/1‑140 (MQ=255)                                                 tCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGc                                                                                        >  2:415315/1‑140 (MQ=255)                                                     tGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCaa                                                                                    >  1:1000485/1‑140 (MQ=255)                                                      gTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAAc                                                                                   <  2:1000485/140‑1 (MQ=255)                                                      gTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAAc                                                                                   <  2:564422/140‑1 (MQ=255)                                                      gTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAAc                                                                                   >  2:855501/1‑140 (MQ=255)                                                           aGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATcc                                                                                          <  2:724736/128‑1 (MQ=255)                                                           aGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATcc                                                                                          >  1:724736/1‑128 (MQ=255)                                                               gAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGttgttg                                                                          >  1:757848/1‑140 (MQ=255)                                                               gAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGttgttg                                                                          <  2:423736/140‑1 (MQ=255)                                                                 aTCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGAc                                                                        <  1:278124/140‑1 (MQ=255)                                                                 aTCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGAc                                                                        <  2:757848/140‑1 (MQ=255)                                                                     gggCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGa                                                                    <  1:504436/140‑1 (MQ=255)                                                                           aaGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATaca                                                              <  1:878010/140‑1 (MQ=255)                                                                           aaGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATaca                                                              <  1:1139382/140‑1 (MQ=255)                                                                             gTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCACAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACg                                                            >  2:555478/1‑140 (MQ=255)                                                                                gCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTg                                                         >  2:97652/1‑140 (MQ=255)                                                                                gCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTg                                                         >  2:1077470/1‑140 (MQ=255)                                                                                gCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTg                                                         >  1:199996/1‑140 (MQ=255)                                                                                gCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCCCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATacac                                                             <  2:79695/136‑1 (MQ=255)                                                                                gCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCCCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATacac                                                             >  1:79695/1‑136 (MQ=255)                                                                                    acTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAg                                                     <  1:415315/140‑1 (MQ=255)                                                                                     cTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGa                                                    >  2:355815/1‑140 (MQ=255)                                                                                     cTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGa                                                    >  2:210775/1‑140 (MQ=255)                                                                                     cTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGa                                                    >  2:902891/1‑140 (MQ=255)                                                                                          tttGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCcg                                               >  2:632150/1‑140 (MQ=255)                                                                                            tGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCcgcg                                             <  2:804105/140‑1 (MQ=255)                                                                                            tGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCcgcg                                             <  1:855501/140‑1 (MQ=255)                                                                                            tGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCcgcg                                             <  2:991601/140‑1 (MQ=255)                                                                                                  ccTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCt                                       >  2:296015/1‑140 (MQ=255)                                                                                                           agGTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAATGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGtt                              >  2:627908/1‑140 (MQ=255)                                                                                                             gTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTgg                            >  1:1034305/1‑140 (MQ=255)                                                                                                             gTGATATGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTgg                            >  2:655981/1‑140 (MQ=255)                                                                                                                atatGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCt                         <  1:902891/140‑1 (MQ=255)                                                                                                                atatGCTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCt                         >  1:291177/1‑140 (MQ=255)                                                                                                                     cTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGc                           <  1:1059690/133‑1 (MQ=255)                                                                                                                     cTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGc                           >  2:1059690/1‑133 (MQ=255)                                                                                                                     cTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCt                         <  1:1070297/135‑1 (MQ=255)                                                                                                                     cTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCt                         >  2:1070297/1‑135 (MQ=255)                                                                                                                     cTCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCca                    >  1:401836/1‑140 (MQ=255)                                                                                                                      tCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGTCTTTCcac                   <  2:199996/140‑1 (MQ=255)                                                                                                                      tCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCcac                   <  1:926919/140‑1 (MQ=255)                                                                                                                      tCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCcac                   <  1:718486/140‑1 (MQ=255)                                                                                                                      tCACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCACAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCcac                   <  1:555478/140‑1 (MQ=255)                                                                                                                       cACCTCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCcaca                  >  1:118846/1‑140 (MQ=255)                                                                                                                           tCAGAACAACACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTTACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGa              >  2:150414/1‑140 (MQ=255)                                                                                                                               aacaacACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAg          >  2:442462/1‑140 (MQ=255)                                                                                                                               aacaacACAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAg          >  1:402481/1‑140 (MQ=255)                                                                                                                                acaacaCAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGa         <  1:210775/140‑1 (MQ=255)                                                                                                                                acaacaCAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGa         <  1:603601/140‑1 (MQ=255)                                                                                                                                  aacaCAGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATg       >  1:414355/1‑140 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGCGTTTAAACGCGAATCCGCTCAACTGGCTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     >  2:316147/1‑140 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     >  1:210636/1‑140 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     >  1:318101/1‑140 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     <  1:655981/140‑1 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     >  2:656095/1‑140 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     >  2:610170/1‑140 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     >  1:960564/1‑140 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     >  2:550055/1‑140 (MQ=255)                                                                                                                                    cacaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATggg     >  2:167316/1‑140 (MQ=255)                                                                                                                                     acaGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGAAGGGt    >  2:1060642/1‑140 (MQ=255)                                                                                                                                       aGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATg       <  1:227014/135‑1 (MQ=255)                                                                                                                                       aGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATg       >  2:227014/1‑135 (MQ=255)                                                                                                                                       aGGCGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCa  >  2:818581/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TTGCTTGTTATTGGTATATTCCTGACGTTCTTCATGCGTCCAGATAAGTCATTGTAAAAGTCGAATCAGGGCTGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGTTTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAAATACACGGTGGCAGATGCCGCGAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGTCA  >  NC_000913/4507053‑4507326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑