Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	432,214	T→C	intergenic (‑201/+98)	tsx ← / ← yajI	nucleoside‑specific channel‑forming protein Tsx/putative lipoprotein YajI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	432,214	0	T	C	100.0%	325.3 / NA	86	intergenic (‑201/+98)	tsx/yajI	nucleoside‑specific channel‑forming protein Tsx/putative lipoprotein YajI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/41);  total (45/41)

GAGGAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACA  >  NC_000913/432089‑432348                                                                                                                              |                                                                                                                                       gaggagCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCtcgttg                                                                                                                                    >  2:1145262/1‑130 (MQ=255) gaggagCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCtcgttg                                                                                                                                    <  1:1145262/130‑1 (MQ=255)  aggagCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTAc                                                                                                                         >  2:185587/1‑140 (MQ=255)  aggagCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTAc                                                                                                                         >  2:505281/1‑140 (MQ=255)  aggagCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTAc                                                                                                                         >  2:706174/1‑140 (MQ=255)     agCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTAt                                                                                                                      >  2:2741/1‑140 (MQ=255)     agCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTAt                                                                                                                      >  2:473283/1‑140 (MQ=255)     agCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTAt                                                                                                                      >  1:312148/1‑140 (MQ=255)     agCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTAt                                                                                                                      >  2:250593/1‑140 (MQ=255)       ccTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCa                                                                                                                    >  2:717401/1‑140 (MQ=255)       ccTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCa                                                                                                                    >  2:704968/1‑140 (MQ=255)         tattatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAAt                                                                                                                  >  1:887174/1‑140 (MQ=255)          attatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACTCACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATc                                                                                                                 >  1:705220/1‑140 (MQ=255)          attatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATc                                                                                                                     >  2:950886/1‑136 (MQ=255)          attatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATc                                                                                                                     <  1:950886/136‑1 (MQ=255)            tataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCa                                                                                                               >  1:988234/1‑140 (MQ=255)             ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAg                                                                                                              <  2:997482/140‑1 (MQ=255)             ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAg                                                                                                              <  2:312148/140‑1 (MQ=255)             ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAg                                                                                                              <  2:217756/140‑1 (MQ=255)             ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAg                                                                                                              <  2:11948/140‑1 (MQ=255)             ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAg                                                                                                              <  1:473283/140‑1 (MQ=255)             ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAg                                                                                                              <  2:887174/140‑1 (MQ=255)             ataAAAATCATTGCTCACAAAAATATGTTTCGTTACACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAg                                                                                                              <  1:649969/140‑1 (MQ=255)              taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACt                                                                                                                        >  1:35902/1‑129 (MQ=255)              taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACt                                                                                                                        <  2:35902/129‑1 (MQ=255)                 aaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGa                                                                                                          >  2:404534/1‑140 (MQ=255)                   aTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATg                                                                                                        >  2:56741/1‑140 (MQ=255)                     cATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTa                                                                                                      <  1:1133030/140‑1 (MQ=255)                       ttGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAg                                                                                                    <  1:56741/140‑1 (MQ=255)                       ttGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAg                                                                                                    <  1:2741/140‑1 (MQ=255)                        tGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGc                                                                                                   >  2:642352/1‑140 (MQ=255)                            cacaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCagag                                                                                               <  1:404534/140‑1 (MQ=255)                                    tatGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACaaaa                                                                                       >  2:286162/1‑140 (MQ=255)                                       gtttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACTCACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGa                                                                                    <  2:705220/140‑1 (MQ=255)                                       gtttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGa                                                                                    <  1:505281/140‑1 (MQ=255)                                       gtttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGa                                                                                    <  1:706174/140‑1 (MQ=255)                                         ttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACg                                                                                  <  2:988234/140‑1 (MQ=255)                                              ttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGaa                                                                             <  1:1044775/140‑1 (MQ=255)                                                     ttCTATCATTTACGTAATAGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGtt                                                                      >  2:57399/1‑140 (MQ=255)                                                       cTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCATTCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGc                                                                    >  1:1108726/1‑140 (MQ=255)                                                          tCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCggg                                                                 >  2:983273/1‑140 (MQ=255)                                                          tCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCggg                                                                 >  1:578105/1‑140 (MQ=255)                                                           cATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGa                                                                <  1:250593/140‑1 (MQ=255)                                                              ttACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGc                                                             >  2:111251/1‑140 (MQ=255)                                                               tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGca                                                            >  1:934691/1‑140 (MQ=255)                                                               tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGca                                                            >  2:732498/1‑140 (MQ=255)                                                               tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGca                                                            >  1:397898/1‑140 (MQ=255)                                                                     aTCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAAt                                                      >  2:1096438/1‑140 (MQ=255)                                                                      tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATc                                                     >  2:244974/1‑140 (MQ=255)                                                                      tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATc                                                     >  2:1064162/1‑140 (MQ=255)                                                                      tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATc                                                     >  2:1122329/1‑140 (MQ=255)                                                                      tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATc                                                     >  2:208072/1‑140 (MQ=255)                                                                            gCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCg                                               >  1:565572/1‑140 (MQ=255)                                                                                  aCGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATAc                                         >  1:1044136/1‑140 (MQ=255)                                                                                  aCGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATAc                                         <  1:208072/140‑1 (MQ=255)                                                                                     tttACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGtt                                      <  2:1044136/140‑1 (MQ=255)                                                                                     tttACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGtt                                      <  1:185587/140‑1 (MQ=255)                                                                                     tttACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGtt                                      <  1:658209/140‑1 (MQ=255)                                                                                     tttACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGtt                                      <  1:111251/140‑1 (MQ=255)                                                                                       tACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTa                                    >  2:280629/1‑140 (MQ=255)                                                                                        acacacATTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGTACAAAATCGTCCCGAAATACGTTTAc                                   >  1:1141959/1‑140 (MQ=255)                                                                                         cacacaTTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCATTCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACt                                  <  2:1108726/140‑1 (MQ=255)                                                                                         cacacaTTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACt                                  >  2:177504/1‑140 (MQ=255)                                                                                         cacacaTTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACt                                  >  1:14101/1‑140 (MQ=255)                                                                                             caTTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCAt                              <  1:286162/140‑1 (MQ=255)                                                                                              aTTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCAt                              >  1:821236/1‑139 (MQ=255)                                                                                              aTTCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCAt                              <  2:821236/139‑1 (MQ=255)                                                                                               ttCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGTACAAAATCGTCCCGAAATACGTTTACTGCATaa                            <  2:1141959/140‑1 (MQ=255)                                                                                                tCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGtt                                      >  1:100694/1‑129 (MQ=255)                                                                                                tCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGtt                                      <  2:100694/129‑1 (MQ=255)                                                                                                tCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAAc                           <  2:119483/140‑1 (MQ=255)                                                                                                tCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAAc                           <  1:939907/140‑1 (MQ=255)                                                                                                tCGGGCGGGGATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAAc                           >  2:438630/1‑140 (MQ=255)                                                                                                       gggATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGa                    <  2:578105/140‑1 (MQ=255)                                                                                                       gggATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGa                    <  2:397898/140‑1 (MQ=255)                                                                                                        ggATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGaa                   <  1:704968/140‑1 (MQ=255)                                                                                                        ggATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGaa                   >  2:415120/1‑140 (MQ=255)                                                                                                        ggATTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGaa                   >  2:44297/1‑140 (MQ=255)                                                                                                          aTTGTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATg                 <  1:438630/140‑1 (MQ=255)                                                                                                             gTACTGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCg              <  1:169992/140‑1 (MQ=255)                                                                                                                 tGACTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGa          <  1:244974/140‑1 (MQ=255)                                                                                                                   aCTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATa        >  1:438783/1‑140 (MQ=255)                                                                                                                   aCTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATa        >  1:530283/1‑140 (MQ=255)                                                                                                                    cTTTCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATAc       >  1:636261/1‑140 (MQ=255)                                                                                                                       tCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTa    <  1:280629/140‑1 (MQ=255)                                                                                                                       tCGCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTa    <  1:642352/140‑1 (MQ=255)                                                                                                                         gCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACa  <  2:636261/140‑1 (MQ=255)                                                                                                                         gCCCTCGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACa  <  1:732498/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                       GAGGAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACA  >  NC_000913/432089‑432348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑