Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	733,427	T→C	V1282A (GTC→GCC)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	733,427	0	T	C	100.0%	323.2 / NA	85	V1282A (GTC→GCC)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/42);  total (43/42)

AATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACC  >  NC_000913/733291‑733563                                                                                                                                         |                                                                                                                                         aaTCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGcccc                                                                                                                                       >  2:986233/1‑140 (MQ=255)  aTCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCa                                                                                                                                      <  1:812026/140‑1 (MQ=255)  aTCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCa                                                                                                                                      >  2:21392/1‑140 (MQ=255)    ccGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGa                                                                                                                                    <  1:358559/140‑1 (MQ=255)         ctcAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTa                                                                                                                               <  1:56131/140‑1 (MQ=255)           cAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTaaa                                                                                                                             <  1:710250/140‑1 (MQ=255)           cAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTaaa                                                                                                                             <  2:262299/140‑1 (MQ=255)            aaaTATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAg                                                                                                                            <  1:234901/140‑1 (MQ=255)              atatAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAggg                                                                                                                          <  2:521898/140‑1 (MQ=255)               tataGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAgggg                                                                                                                         <  1:563106/140‑1 (MQ=255)               tataGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAgggg                                                                                                                         <  1:55846/140‑1 (MQ=255)                ataGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGa                                                                                                                        <  2:942203/140‑1 (MQ=255)                    aTCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATTGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACtat                                                                                                                    >  1:79269/1‑140 (MQ=255)                    aTCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACtat                                                                                                                    >  2:645837/1‑140 (MQ=255)                         ttAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAg                                                                                                                            <  2:818397/127‑1 (MQ=255)                         ttAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAg                                                                                                                            >  1:818397/1‑127 (MQ=255)                         ttAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTtg                                                                                                               <  2:155340/140‑1 (MQ=255)                         ttAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGGAAAGGGGACTATACTtg                                                                                                               >  2:1026479/1‑140 (MQ=255)                         ttAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAAT‑GGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTtgt                                                                                                              >  2:210352/1‑140 (MQ=255)                              aTTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTtgtg                                                                                                             <  1:288388/137‑1 (MQ=255)                              aTTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTtgtg                                                                                                             >  2:288388/1‑137 (MQ=255)                                 aGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTtgtg                                                                                                             <  2:756139/134‑1 (MQ=255)                                 aGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTtgtg                                                                                                             >  1:756139/1‑134 (MQ=255)                                 aGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGcc                                                                                                       <  1:1080366/140‑1 (MQ=255)                                 aGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCAACAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGcc                                                                                                       >  2:931302/1‑140 (MQ=255)                                     acacTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGa                                                                                                   <  1:986233/140‑1 (MQ=255)                                       acTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGcc                                                                                                       <  1:505969/134‑1 (MQ=255)                                       acTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGcc                                                                                                       >  2:505969/1‑134 (MQ=255)                                       acTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGAcc                                                                                                 >  1:822080/1‑140 (MQ=255)                                       acTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGAAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGAcc                                                                                                 >  2:975476/1‑140 (MQ=255)                                         tAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCaa                                                                                               >  1:1122912/1‑140 (MQ=255)                                              tGCATTAAGCCACTGCATTCAAT‑GGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGga                                                                                         <  1:210352/140‑1 (MQ=255)                                               gCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGga                                                                                         <  2:822080/140‑1 (MQ=255)                                               gCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGga                                                                                         <  1:1026479/140‑1 (MQ=255)                                               gCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGga                                                                                         <  1:246364/140‑1 (MQ=255)                                                         aCTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCAACAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAg                                                                               <  1:931302/140‑1 (MQ=255)                                                          cTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAgg                                                                              >  2:914568/1‑140 (MQ=255)                                                           tGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGga                                                                             >  2:462641/1‑140 (MQ=255)                                                           tGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGCACTATACTTGTGGTGGCCAAGACCAACGGGGAGAAt                                                                                     >  2:793377/1‑132 (MQ=255)                                                           tGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGCACTATACTTGTGGTGGCCAAGACCAACGGGGAGAAt                                                                                     <  1:793377/132‑1 (MQ=255)                                                            gCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATTGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGgag                                                                            <  2:79269/140‑1 (MQ=255)                                                             cATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGgaga                                                                           <  1:259147/140‑1 (MQ=255)                                                             cATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGgag                                                                            <  2:341552/139‑1 (MQ=255)                                                             cATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGgag                                                                            >  1:341552/1‑139 (MQ=255)                                                               ttCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCTAGACCAACGGGGAGAATCAAAAGGAGATg                                                                         >  1:14070/1‑140 (MQ=255)                                                                 cAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGt                                                                       <  1:914568/140‑1 (MQ=255)                                                                  aaTGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTc                                                                      >  2:918337/1‑140 (MQ=255)                                                                     gggCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGTGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGAGGCCAAGTCCAACGGGGAGAATCAAAAGGAGATGGTCTAt                                                                   >  2:1017973/1‑140 (MQ=255)                                                                       gCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATgg                                                                 >  1:346545/1‑140 (MQ=255)                                                                              tGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAg                                                          <  1:462641/140‑1 (MQ=255)                                                                                gTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGt                                                                       <  1:12400/125‑1 (MQ=255)                                                                                gTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGt                                                                       >  2:12400/1‑125 (MQ=255)                                                                                gTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGt                                                        >  2:803995/1‑140 (MQ=255)                                                                                     agaagCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGc                                                             <  1:931757/130‑1 (MQ=255)                                                                                     agaagCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGc                                                             >  2:931757/1‑130 (MQ=255)                                                                                      gaagCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCa                                                  <  1:803995/140‑1 (MQ=255)                                                                                      gaagCGGTCCAGATATATGGGGAAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCa                                                  <  1:975476/140‑1 (MQ=255)                                                                                        agCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAg                                                <  1:675238/140‑1 (MQ=255)                                                                                        agCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAg                                                >  1:111119/1‑140 (MQ=255)                                                                                             tCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATg                                           >  2:974643/1‑140 (MQ=255)                                                                                             tCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATg                                           >  1:188167/1‑140 (MQ=255)                                                                                              ccAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGa                                          <  1:918337/140‑1 (MQ=255)                                                                                                      atGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACaaa                                    <  2:618624/138‑1 (MQ=255)                                                                                                      atGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACaaa                                    >  1:618624/1‑138 (MQ=255)                                                                                                       tGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAaaga                                 >  1:435786/1‑140 (MQ=255)                                                                                                       tGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAaaga                                 <  2:346545/140‑1 (MQ=255)                                                                                                       tGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAaaga                                 >  2:436266/1‑140 (MQ=255)                                                                                                       tGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAaaga                                 >  2:185498/1‑140 (MQ=255)                                                                                                       tGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAaaga                                 >  2:578798/1‑140 (MQ=255)                                                                                                       tGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAaaga                                 >  1:468570/1‑140 (MQ=255)                                                                                                       tGGGGGAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAaaga                                 >  1:804023/1‑140 (MQ=255)                                                                                                         ggggAATCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAaagaag                               >  2:192639/1‑140 (MQ=255)                                                                                                             aaTCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCTAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAgctgc                           <  2:14070/140‑1 (MQ=255)                                                                                                               tCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGctgctg                         >  2:33816/1‑140 (MQ=255)                                                                                                               tCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGctgctg                         >  1:1063846/1‑140 (MQ=255)                                                                                                               tCCGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGctgctg                         >  1:768509/1‑140 (MQ=255)                                                                                                                ccGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTgg                        <  1:402682/140‑1 (MQ=255)                                                                                                                 cGTTCTATCATCAATATCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGc                       <  1:578798/140‑1 (MQ=255)                                                                                                                                atCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTgg                        >  1:900297/1‑124 (MQ=255)                                                                                                                                atCTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTgg                        <  2:900297/124‑1 (MQ=255)                                                                                                                                  cTTTGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGa      >  2:224649/1‑140 (MQ=255)                                                                                                                                   tttGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGaa     <  2:768509/140‑1 (MQ=255)                                                                                                                                    ttGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGaaa    >  2:320338/1‑140 (MQ=255)                                                                                                                                    ttGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGaaa    >  1:213438/1‑140 (MQ=255)                                                                                                                                    ttGTGCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGaaa    <  1:436266/140‑1 (MQ=255)                                                                                                                                     tgtgCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAAc   >  2:976922/1‑140 (MQ=255)                                                                                                                                     tgtgCCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAAc   >  1:103889/1‑140 (MQ=255)                                                                                                                                      gtgcCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGaaa    <  1:343009/138‑1 (MQ=255)                                                                                                                                      gtgcCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAAcc  <  1:33816/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         AATCCGATCTCAAATATAGATCCATTAGGATTAGAAACACTAAAATGCATTAAGCCACTGCATTCAATGGGCGGAACTGGTGAAAGAAGCGGTCCAGATATATGGGGGAATCCGTTCTATCATCAATATCTTTGTGTCCCAGATGGTAAAGGGGACTATACTTGTGGTGGCCAAGACCAACGGGGAGAATCAAAAGGAGATGGTCTATGGGGGCCAGGTAAAGCAAGTAATGATACAAAAGAAGCTGCTGGCCGTTGTGACCTCGTTGAAACC  >  NC_000913/733291‑733563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑