Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	778,178	A→G	T147A (ACC→GCC)	tolB →	Tol‑Pal system periplasmic protein TolB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	778,178	0	A	G	100.0%	316.9 / NA	84	T147A (ACC→GCC)	tolB	Tol‑Pal system periplasmic protein TolB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/43);  total (41/43)

GGTCAGGTCACTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAA  >  NC_000913/778043‑778311                                                                                                                                        |                                                                                                                                      ggtcaggtcaCTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGGACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGc                                                                                                                                   >  2:272695/1‑140 (MQ=255) ggtcaggtcaCTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGc                                                                                                                                   <  1:521774/140‑1 (MQ=255) ggtcaggtcaCTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGc                                                                                                                                   <  1:29313/140‑1 (MQ=255) agtcaggtcaCTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGc                                                                                                                                   >  1:1083704/2‑140 (MQ=255)   tcaggtcaCTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCa                                                                                                                                 >  2:333847/1‑140 (MQ=255)   tcaggtcaCTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCa                                                                                                                                 >  2:635887/1‑140 (MQ=255)       gtcaCTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGtga                                                                                                                             >  1:564526/1‑140 (MQ=255)         caCTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGtgatg                                                                                                                           <  2:108087/140‑1 (MQ=255)             ccGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAgt                                                                                                                       <  2:439948/140‑1 (MQ=255)                aaTCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGtt                                                                                                                    >  1:89013/1‑140 (MQ=255)                 aTCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGtgatg                                                                                                                           <  2:413526/132‑1 (MQ=255)                 aTCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGtgatg                                                                                                                           >  1:413526/1‑132 (MQ=255)                 aTCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGttt                                                                                                                   >  1:818823/1‑140 (MQ=255)                 aTCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGttt                                                                                                                   >  1:513864/1‑140 (MQ=255)                 aTCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGttt                                                                                                                   >  2:1143587/1‑140 (MQ=255)                      gATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGaaaa                                                                                                              >  1:137067/1‑140 (MQ=255)                            tCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAGCTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGAc                                                                                                        >  1:635348/1‑140 (MQ=255)                             cTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGAcc                                                                                                       <  2:145188/140‑1 (MQ=255)                             cTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGAcc                                                                                                       >  2:653917/1‑140 (MQ=255)                             cTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGAcc                                                                                                       <  2:137067/140‑1 (MQ=255)                             cTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGAcc                                                                                                       <  1:1143587/140‑1 (MQ=255)                             cTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGAcc                                                                                                       <  2:818823/140‑1 (MQ=255)                             cTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGAcc                                                                                                       <  1:61092/140‑1 (MQ=255)                             cTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGAcc                                                                                                       <  1:653917/140‑1 (MQ=255)                                aCAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGGACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGc                                                                                                    <  1:272695/140‑1 (MQ=255)                                aCAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGc                                                                                                    >  1:1025438/1‑140 (MQ=255)                                    tGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTa                                                                                                <  1:58949/140‑1 (MQ=255)                                     gTTGCTTATCAACTTGTTGACACTGGCGGCGCAACGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTaa                                                                                               >  1:529078/1‑140 (MQ=255)                                      ttGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTaaa                                                                                              >  1:275529/1‑140 (MQ=255)                                              cAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCAt                                                                                                  <  2:937426/128‑1 (MQ=255)                                              cAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCAt                                                                                                  >  1:937426/1‑128 (MQ=255)                                              cAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGtt                                                                                      >  2:814058/1‑140 (MQ=255)                                               aaCTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTc                                                                                     <  1:282117/140‑1 (MQ=255)                                                  ttgttgACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGt                                                                                  <  2:1083704/140‑1 (MQ=255)                                                   tgttgACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTa                                                                                 <  2:564526/140‑1 (MQ=255)                                                   tgttgACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTa                                                                                 <  1:333847/140‑1 (MQ=255)                                                       gACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTc                                                                                     <  1:103453/132‑1 (MQ=255)                                                       gACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTc                                                                                     >  2:103453/1‑132 (MQ=255)                                                        acacTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGt                                                                            <  2:56034/140‑1 (MQ=255)                                                              gcggcgCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCg                                                                                        <  2:95829/122‑1 (MQ=255)                                                              gcggcgCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCg                                                                                        >  1:95829/1‑122 (MQ=255)                                                              gcggcgCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGcc                                                                      >  1:718738/1‑140 (MQ=255)                                                               cggcgCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCt                                                                     >  2:886215/1‑140 (MQ=255)                                                                  cgcACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACg                                                                  <  1:1070873/140‑1 (MQ=255)                                                                       cGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACgttgtt                                                             <  2:529078/140‑1 (MQ=255)                                                                                 actTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAAcg                                                   >  2:627688/1‑140 (MQ=255)                                                                                  ctTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAAcgg                                                  >  2:417516/1‑140 (MQ=255)                                                                                    tGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAAcggcg                                                <  2:459519/140‑1 (MQ=255)                                                                                    tGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAAcggcg                                                <  2:53308/140‑1 (MQ=255)                                                                                     gCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAAcggcgg                                               >  1:617294/1‑140 (MQ=255)                                                                                         aGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGtaag                                           <  1:627688/140‑4 (MQ=255)                                                                                         aGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAg                                           <  2:718738/140‑1 (MQ=255)                                                                                         aGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAg                                           >  2:57957/1‑140 (MQ=255)                                                                                          gAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGt                                              >  2:968358/1‑136 (MQ=255)                                                                                          gAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGt                                              <  1:968358/136‑1 (MQ=255)                                                                                          gAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGt                                          >  1:91427/1‑140 (MQ=255)                                                                                          gAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGt                                          >  1:588091/1‑140 (MQ=255)                                                                                           agcTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGtt                                         <  2:635348/138‑1 (MQ=255)                                                                                           aaCTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCTGTACTCGTATTGCCTACGTTGTTCAGTCCAACGGCGGTCAGtt                                         <  2:1025438/140‑1 (MQ=255)                                                                                           aaCTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGtt                                         <  2:1071432/140‑1 (MQ=255)                                                                                                gTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGt                                    >  2:478284/1‑140 (MQ=255)                                                                                                gTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGt                                    >  1:382098/1‑140 (MQ=255)                                                                                                 tACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTa                                   <  1:635887/140‑1 (MQ=255)                                                                                                      aGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGtt                                         <  2:871977/129‑1 (MQ=255)                                                                                                      aGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGtt                                         >  1:871977/1‑129 (MQ=255)                                                                                                       gTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGtt                                         <  2:820601/128‑1 (MQ=255)                                                                                                       gTGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGtt                                         >  1:820601/1‑128 (MQ=255)                                                                                                        tGAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGACAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTg                            >  2:121851/1‑140 (MQ=255)                                                                                                         gAACAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTgc                           <  2:787833/140‑1 (MQ=255)                                                                                                          aaCAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTgcg                          <  2:617294/140‑1 (MQ=255)                                                                                                          aaCAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTgcg                          >  2:36298/1‑140 (MQ=255)                                                                                                          aaCAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTgcg                          <  1:478284/140‑1 (MQ=255)                                                                                                           aCAAGCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTgcgc                         <  2:89013/140‑1 (MQ=255)                                                                                                               gCAGTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTAt                     <  2:91427/140‑1 (MQ=255)                                                                                                                  gTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTg                  >  2:423257/1‑140 (MQ=255)                                                                                                                  gTGGCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATG‑ACTGCGCGTATCTGa                 >  2:536444/1‑140 (MQ=255)                                                                                                                     gCTGCGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACt               >  1:1037588/1‑140 (MQ=255)                                                                                                                         cGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTAt                     <  2:976084/130‑1 (MQ=255)                                                                                                                         cGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTAt                     >  1:976084/1‑130 (MQ=255)                                                                                                                         cGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGa           <  2:588091/140‑1 (MQ=255)                                                                                                                         cGTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGa           >  2:500285/1‑140 (MQ=255)                                                                                                                          gTTATGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGAc          >  2:96829/1‑140 (MQ=255)                                                                                                                             aTGCTGGTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGt       <  1:814058/140‑1 (MQ=255)                                                                                                                                  ggTCATGCCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACaa  <  2:513864/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GGTCAGGTCACTCCGAATCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGTACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAAGGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTATGACGGTTACAA  >  NC_000913/778043‑778311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑