Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,165,817	A→G	intergenic (+132/‑268)	ycfP → / → ndh	UPF0227 protein YcfP/NADH:quinone oxidoreductase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,165,817	0	A	G	100.0%	201.6 / NA	55	intergenic (+132/‑268)	ycfP/ndh	UPF0227 protein YcfP/NADH:quinone oxidoreductase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/21);  total (34/21)

GGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTT  >  NC_000913/1165681‑1165952                                                                                                                                         |                                                                                                                                        ggTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAgcgc                                                                                                                                      <  1:960875/140‑1 (MQ=255) ggTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAgcgc                                                                                                                                      <  2:234201/140‑1 (MQ=255)      aTGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGc                                                                                                                                 >  2:681927/1‑140 (MQ=255)       tGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCa                                                                                                                                >  1:277236/1‑140 (MQ=255)        gCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCAt                                                                                                                               <  1:964219/140‑1 (MQ=255)        gCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCAt                                                                                                                               <  1:681927/140‑1 (MQ=255)          ccTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATac                                                                                                                             <  1:872236/140‑1 (MQ=255)            tcgtcgCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATacac                                                                                                                           >  1:427532/1‑140 (MQ=255)                cgCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCt                                                                                                                       <  1:1064524/140‑1 (MQ=255)                   aTCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCAc                                                                                                                    <  2:522960/140‑1 (MQ=255)                    tCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCAct                                                                                                                   <  2:277236/140‑1 (MQ=255)                             cTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCAc                                                                                                          <  1:311511/140‑1 (MQ=255)                             cTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCAc                                                                                                          >  1:270440/1‑140 (MQ=255)                             cTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCAc                                                                                                          >  1:253585/1‑140 (MQ=255)                               tGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCActc                                                                                                        >  1:472717/1‑140 (MQ=255)                                gCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCActct                                                                                                       >  2:136175/1‑140 (MQ=255)                                      aCCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACaaa                                                                                                 >  1:324498/1‑140 (MQ=255)                                               gcTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCaa                                                                                        >  2:806665/1‑140 (MQ=255)                                               gcTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCaa                                                                                        >  1:126897/1‑140 (MQ=255)                                                cTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTc                                                                                          >  1:5088/1‑137 (MQ=255)                                                cTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTc                                                                                          <  2:5088/137‑1 (MQ=255)                                                cTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCaaa                                                                                       >  2:676268/1‑140 (MQ=255)                                                cTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCaaa                                                                                       >  2:991451/1‑140 (MQ=255)                                                  ccGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAaata                                                                                     >  2:620888/1‑140 (MQ=255)                                                  ccGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAaata                                                                                     <  1:991451/140‑1 (MQ=255)                                                    gAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCTACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAaataat                                                                                   <  2:532500/140‑1 (MQ=255)                                                             gggTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaacaataa                                                                          >  1:737724/1‑140 (MQ=255)                                                             gggTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaacaataa                                                                          >  2:1071263/1‑140 (MQ=255)                                                               gTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATaataaacaataaac                                                                        >  1:308105/1‑140 (MQ=255)                                                                tCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAAct                                                                       <  2:737724/140‑1 (MQ=255)                                                                   gATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTg                                                                    >  2:554532/1‑140 (MQ=255)                                                                    aTAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGt                                                                   >  1:520543/1‑140 (MQ=255)                                                                       aGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGtttt                                                                <  2:270440/140‑1 (MQ=255)                                                                       aGGCGTCCACGCCGCATCCGACAGTCCAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGtttt                                                                <  1:806665/140‑1 (MQ=255)                                                                            tCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGAt                                                           <  1:620888/140‑1 (MQ=255)                                                                            tCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCGGTTTTTTGAt                                                           >  1:790199/1‑140 (MQ=255)                                                                             ccACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGAtc                                                          >  2:1128127/1‑140 (MQ=255)                                                                             ccACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGAtc                                                          >  2:830474/1‑140 (MQ=255)                                                                             ccACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGAtc                                                          >  2:687481/1‑140 (MQ=255)                                                                             ccACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGAtc                                                          >  2:310544/1‑140 (MQ=255)                                                                              cACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGAt                                                           <  1:347903/138‑1 (MQ=255)                                                                              cACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGAt                                                           >  2:347903/1‑138 (MQ=255)                                                                                       tCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTa                                                <  1:554532/140‑1 (MQ=255)                                                                                             aGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCg                                          <  2:253585/140‑1 (MQ=255)                                                                                             aGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAACTCGCTCAAATAATAAACAATAAACCCTGTTTTTTGATCTCACCCGGTAAAGTCg                                          >  1:1037880/1‑140 (MQ=255)                                                                                                 gAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTa                                      >  2:366003/1‑140 (MQ=255)                                                                                                  aGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTAt                                     >  2:729575/1‑140 (MQ=255)                                                                                                             cTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGcaa                          <  2:520543/140‑1 (MQ=255)                                                                                                             cTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGcaa                          >  2:85818/1‑140 (MQ=255)                                                                                                             cTGATGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGcaa                          <  1:932427/140‑1 (MQ=255)                                                                                                                 tGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACaaa                      >  2:27554/1‑140 (MQ=255)                                                                                                                 tGCGCTTCTTATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACaaa                      >  1:754893/1‑140 (MQ=255)                                                                                                                      ttcttATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTg                 >  2:531354/1‑140 (MQ=255)                                                                                                                        cttATCAGGCCTACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGAt               <  1:830474/140‑1 (MQ=255)                                                                                                                                   tACCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAAtt    <  1:627/140‑1 (MQ=255)                                                                                                                                    aCCGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAAttt   >  2:140595/1‑140 (MQ=255)                                                                                                                                     ccGAGCGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAAtttt  >  2:196068/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GGTAAATGCCCTCGTCGCATCAGGTAACCTTGCCGGTACCTGATGCGCTCCGAATTCTGTGGGTCGGATAAGGCGTCCACGCCGCATCCGACAGTCGAGCATCAATGCCTGATGCGCTTCTTATCAGGCCTACCGAACGCCCTGCATACACCCCTCACTCTATATCACTCTCACAAATTCGCTCAAATAATAAACAATAAACTCTGTTTTTTGATCTCACCCGGTAAAGTCGCCTATCTTTTCAGCAACAAAACTTGATTAACATCAATTTT  >  NC_000913/1165681‑1165952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑