BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	81,219	T→C	N247S (AAC→AGC)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	81,219	0	T	C	100.0%	374.4 / NA	99	N247S (AAC→AGC)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (47/52); total (47/52)

CTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAAT  >  NC_000913/81085‑81352

cTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAg                                                                                                                                  <  2:642745/140‑1 (MQ=255)

cTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAg                                                                                                                                  <  1:258039/140‑1 (MQ=255)

  gCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGca                                                                                                                                >  2:265540/1‑140 (MQ=255)

  gCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGca                                                                                                                                <  1:243129/140‑1 (MQ=255)

       atCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCAcagca                                                                                                                           <  2:256582/140‑1 (MQ=255)

       atCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCAcagca                                                                                                                           <  2:657358/140‑1 (MQ=255)

       atCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCAcagca                                                                                                                           <  1:265540/140‑1 (MQ=255)

             tGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACATCCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACg                                                                                                                      >  1:589713/1‑140 (MQ=255)

             tGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGt                                                                                                                     >  1:573826/1‑140 (MQ=255)

             tGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGt                                                                                                                     >  2:52404/1‑140 (MQ=255)

               ccGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCa                                                                                                                   <  2:59995/140‑1 (MQ=255)

                cGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCaa                                                                                                                  >  1:596761/1‑140 (MQ=255)

                  aGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAAc                                                                                                                >  2:465904/1‑140 (MQ=255)

                  aGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAAc                                                                                                                >  2:357382/1‑140 (MQ=255)

                  aGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAAc                                                                                                                >  1:510204/1‑140 (MQ=255)

                    ccgcccgcCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACtg                                                                                                              >  2:351113/1‑140 (MQ=255)

                      gcccgccGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACtgtg                                                                                                            >  1:1055497/1‑140 (MQ=255)

                       cccgccGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGa                                                                                                           >  1:167027/1‑140 (MQ=255)

                         cgccgGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATg                                                                                                         <  1:351113/140‑1 (MQ=255)

                                 cATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑TCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTa                                                                                                 >  2:594460/1‑140 (MQ=255)

                                 cATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTa                                                                                                 <  1:584273/140‑1 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGa                                                                                                           >  1:754757/1‑123 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGa                                                                                                           <  2:754757/123‑1 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATg                                                                                                         >  1:998522/1‑125 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATg                                                                                                         <  2:998522/125‑1 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTa                                                                                                 >  2:179033/1‑133 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTa                                                                                                 <  1:179033/133‑1 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAAt                                                                                               >  2:222227/1‑135 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAAt                                                                                               <  1:222227/135‑1 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATc                                                                                              <  2:319212/136‑1 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATc                                                                                              >  1:319212/1‑136 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt                                                                                          >  2:677863/1‑140 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt                                                                                          <  2:1081854/140‑1 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt                                                                                          >  2:943257/1‑140 (MQ=255)

                                        tCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCt                                                                                          >  1:431828/1‑140 (MQ=255)

                                                  tGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGc                                                                                >  2:915929/1‑140 (MQ=255)

                                                  tGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGc                                                                                >  1:587334/1‑140 (MQ=255)

                                                  tGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGc                                                                                >  2:758537/1‑140 (MQ=255)

                                                  tGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGc                                                                                >  1:15512/1‑140 (MQ=255)

                                                  tGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGc                                                                                >  2:47340/1‑140 (MQ=255)

                                                  tGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGc                                                                                >  2:225629/1‑140 (MQ=255)

                                                  tGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTAAATCAGCTGCATGGTGGc                                                                                >  2:882115/1‑140 (MQ=255)

                                                   gCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                                                               <  2:15512/140‑1 (MQ=255)

                                                   gCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                                                               <  2:167027/140‑1 (MQ=255)

                                                   gCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCg                                                                               <  2:245803/140‑1 (MQ=255)

                                                    cTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGt                                                                              <  1:915929/140‑1 (MQ=255)

                                                    cTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGt                                                                              <  2:59624/140‑1 (MQ=255)

                                                    cTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGt                                                                              <  1:624588/140‑1 (MQ=255)

                                                    cTCGTTCAGGCTGGCGGAAAGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGt                                                                              <  2:942861/140‑1 (MQ=255)

                                                     tCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGtt                                                                             <  1:758537/140‑1 (MQ=255)

                                                      cGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTg                                                                            <  2:571294/140‑1 (MQ=255)

                                                      cGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTg                                                                            <  2:473245/140‑1 (MQ=255)

                                                       gTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATGATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt                                                                           <  2:1069694/140‑1 (MQ=255)

                                                       gTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt                                                                           <  2:573826/140‑1 (MQ=255)

                                                       gTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt                                                                           <  1:677863/140‑1 (MQ=255)

                                                       gTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt                                                                           <  1:225629/140‑1 (MQ=255)

                                                        ttCAGGCTGGCGGAAGGCAACATCCCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt                                                                           <  2:589713/140‑1 (MQ=255)

                                                        ttCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTc                                                                          <  1:357382/140‑1 (MQ=255)

                                                         tCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCg                                                                         <  1:465904/140‑1 (MQ=255)

                                                             gCTGGCGGAAGGCAACAT‑TCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                                                                     <  1:594460/140‑1 (MQ=255)

                                                             gCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                                                                     <  2:141420/140‑1 (MQ=255)

                                                             gCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                                                                     <  2:587334/140‑1 (MQ=255)

                                                             gCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGt                                                                     <  1:246295/140‑1 (MQ=255)

                                                                  cGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACata                                                                >  1:996227/1‑140 (MQ=255)

                                                                       ggCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt                                                                           >  1:672222/1‑124 (MQ=255)

                                                                       ggCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGt                                                                           <  2:672222/124‑1 (MQ=255)

                                                                            cAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGtt                                                      <  2:985522/140‑1 (MQ=255)

                                                                                ccccATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGa                                                   <  2:312258/140‑1 (MQ=255)

                                                                                ccccATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGa                                                   <  2:861149/140‑1 (MQ=255)

                                                                                  ccATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACa                                                 >  1:877812/1‑140 (MQ=255)

                                                                                    aTCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATc                                               <  2:949036/140‑1 (MQ=255)

                                                                                     tCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATc                                               <  1:1034304/139‑1 (MQ=255)

                                                                                     tCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATc                                               >  2:1034304/1‑139 (MQ=255)

                                                                                         gCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGt                                          <  2:996227/140‑1 (MQ=255)

                                                                                         gCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGt                                          >  1:56916/1‑140 (MQ=255)

                                                                                              tGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTcc                                     >  1:510730/1‑140 (MQ=255)

                                                                                                 tCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTg                                  <  2:56916/140‑1 (MQ=255)

                                                                                                   aTTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGc                                >  2:739059/1‑140 (MQ=255)

                                                                                                   aTTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGCACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGc                                >  2:530251/1‑140 (MQ=255)

                                                                                                     tGCGCACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGa                              >  2:189085/1‑140 (MQ=255)

                                                                                                      gcgcACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             <  1:530251/140‑1 (MQ=255)

                                                                                                      gcgcACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             <  1:52404/140‑1 (MQ=255)

                                                                                                      gcgcACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             <  1:47340/140‑1 (MQ=255)

                                                                                                       cgcACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAtc                            <  1:739059/140‑1 (MQ=255)

                                                                                                       cgcACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             >  1:1008872/1‑139 (MQ=255)

                                                                                                       cgcACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             <  2:1008872/139‑1 (MQ=255)

                                                                                                        gcACTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAtct                           >  2:535292/1‑140 (MQ=255)

                                                                                                           cTCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGt                        >  1:671515/1‑140 (MQ=255)

                                                                                                            tCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             >  1:509263/1‑134 (MQ=255)

                                                                                                            tCGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             <  2:509263/134‑1 (MQ=255)

                                                                                                             cGTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTa                      >  1:872176/1‑140 (MQ=255)

                                                                                                              gTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCg                               >  2:720455/1‑130 (MQ=255)

                                                                                                              gTCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCg                               <  1:720455/130‑1 (MQ=255)

                                                                                                               tCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             <  2:745377/131‑1 (MQ=255)

                                                                                                               tCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGAt                             >  1:745377/1‑131 (MQ=255)

                                                                                                               tCAGACAGAATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAAc                    >  1:614849/1‑140 (MQ=255)

                                                                                                                      gaATGTCGCCAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTcc             >  2:584657/1‑140 (MQ=255)

                                                                                                                               cAAACAGGCTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATaaa    >  1:1020090/1‑140 (MQ=255)

                                                                                                                                 aaCAGGCTGGAGCACAGTAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTAGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAAt  >  1:973716/1‑140 (MQ=255)

CTGCGATATCTGGTGCCGAGCCGCCCGCCGGTTCATACAGTCCAAAACCTTGCTCGTTCAGGCTGGCGGAAGGCAACAT‑CCCCATCGAGCCAGTGATCATTGCGCACTCGTCAGACAGAATGTCGCCAAACAGGTTGGAGCACAGCAGAACGTCAAACTGTGATGGATCTTTAATCAGCTGCATGGTGGCGTTGTCGATGTACATATGCGCCAGTTCGACATCCGGGTATTCCGTGGCGATCTCGTTAACGATCTCCCGCCATAAAAT  >  NC_000913/81085‑81352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`