Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,738	A→G	C92C (TGT→TGC)	ydhX ←	putative 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,738	0	A	G	100.0%	171.7 / NA	46	C92C (TGT→TGC)	ydhX	putative 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/22);  total (24/22)

GCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAA  >  NC_000913/1750616‑1750861                                                                                                                           |                                                                                                                            gcgcCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGaaaaaa                                                                                                            >  1:3515/1‑140 (MQ=255) gcgcCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGaaaaaa                                                                                                            >  2:568497/1‑140 (MQ=255)       aTGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATa                                                                                                      <  2:736276/140‑1 (MQ=255)       aTGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATa                                                                                                      <  1:27811/140‑1 (MQ=255)       aTGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATa                                                                                                      >  1:450996/1‑140 (MQ=255)        tGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATAc                                                                                                     <  2:640110/140‑1 (MQ=255)                 tGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCg                                                                                            <  2:3515/140‑1 (MQ=255)                   cAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGtt                                                                                          >  2:421536/1‑140 (MQ=255)                    aGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTg                                                                                         >  2:614442/1‑140 (MQ=255)                        gATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGt                                                                                     >  1:1061220/1‑140 (MQ=255)                        gATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGt                                                                                     >  1:1017828/1‑140 (MQ=255)                         aTGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGtt                                                                                    >  2:606162/1‑140 (MQ=255)                         aTGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTAATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGtt                                                                                    >  2:992448/1‑140 (MQ=255)                          tGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTa                                                                                   <  2:920253/140‑1 (MQ=255)                           gCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTAt                                                                                  >  2:359442/1‑140 (MQ=255)                            cACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATc                                                                                 <  1:359442/140‑1 (MQ=255)                             aCTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCg                                                                                <  1:1097384/140‑1 (MQ=255)                                gCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTg                                                                             >  1:901394/1‑140 (MQ=255)                                  gATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGAc                                                                           <  1:127531/140‑1 (MQ=255)                                   aTTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACt                                                                          <  2:438349/140‑1 (MQ=255)                                        tCTACCCGCACGATCCCCTGCTAATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAAt                                                                     <  1:992448/140‑1 (MQ=255)                                          tACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAAtgt                                                                   <  1:1019020/140‑1 (MQ=255)                                          tACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAAtgt                                                                   <  1:568497/140‑1 (MQ=255)                                             ccGCACGATCCCCTGCTCATCACGTCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAAtgtgtg                                                                <  1:470356/140‑1 (MQ=255)                                                cACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCGTGAATGACTAAAAAAAGGATACTGCGTTTCGTTGTCGTTATCGGTGCCTGGAATGTGTGTTa                                                             >  2:746990/1‑140 (MQ=255)                                                          tGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACg                                                   <  2:321277/140‑1 (MQ=255)                                                           gCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACgg                                                  >  2:156063/1‑140 (MQ=255)                                                               atcaCGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTc                                              >  2:1144001/1‑140 (MQ=255)                                                               atcaCGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTc                                              >  2:263348/1‑140 (MQ=255)                                                               atcaCGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTc                                              >  2:564781/1‑140 (MQ=255)                                                                tcaCGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTcc                                             <  1:787536/140‑1 (MQ=255)                                                                 caCGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTccc                                            >  1:872405/1‑140 (MQ=255)                                                                 caCGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTccc                                            >  1:1123186/1‑140 (MQ=255)                                                                 caCGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTccc                                            >  1:452407/1‑140 (MQ=255)                                                                 caCGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTccc                                            >  1:469792/1‑140 (MQ=255)                                                                      cACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGc                                       >  2:468834/1‑140 (MQ=255)                                                                                 cGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGt                            <  1:606162/140‑1 (MQ=255)                                                                                          aaCGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTAc                   >  1:514052/1‑140 (MQ=255)                                                                                           aCGTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACg                  >  2:267541/1‑140 (MQ=255)                                                                                             gTCGATGCACGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGc                <  2:1017828/140‑1 (MQ=255)                                                                                                      cGGAGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCggg       <  2:514052/140‑1 (MQ=255)                                                                                                       ggAGCATCTTCGCAGTGCTGGCATGACTCGCGAAAAAAGTGATACTGCGTTCCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGgc      <  1:746990/140‑1 (MQ=255)                                                                                                         aGCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGgcgc    >  1:876940/1‑140 (MQ=255)                                                                                                          gCATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCa   <  2:450996/140‑1 (MQ=255)                                                                                                           cATCTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCaa  <  1:263348/140‑1 (MQ=255)                                                                                                              cTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCa   <  1:125286/136‑1 (MQ=255)                                                                                                              cTTCGCAGTGCTGGCATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCa   >  2:125286/1‑136 (MQ=255)                                                                                                                           |                                                                                                                            GCGCCAATGCAATAACTGCAGCCGATGCACTGCGATTTTTCTACCCGCACGATCCCCTGCTCATCACGCCACGACGCCCCCGTCGGGCAAACGTCGATGCACGGAGCATCTTCGCAGTGCTGACATGACTGGCGAAAAAAGTGATACTGCGTTTCGTTGTCGTTATCGGTGACTGGAATGTGTGCTATCGATAAACGGCTTCCCTGAGCGGGGACGTGGTTAGTTTTACGGCATGCGCGGGCGCAA  >  NC_000913/1750616‑1750861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑